FFPE human mesothelioma sections stained with 100 ul anti-Wilms Tumor 1 (clone WT1/1434R) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm?s Tumor Recombinant Rabbit Monoclonal Antibody (WT1/1434R).

Anti-Wilm's Tumor 1 Antibody Clone WT1/1434R

Name: Anti-Wilm's Tumor 1 Antibody Clone WT1/1434R
Brand: enQuire Bio
Price: 199 USD
Availability: InStock

$ 199.00 – $ 549.00

Please Select Product Options Below To View The Catalog Number.

SKU: 7490-RBM4
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rabbit IgG kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
7490-RBM4-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
7490-RBM4-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
7490-RBM4-P2	Size: 200 ug, Tag: Unconjugated	$ 549.00

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In Stock.

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 7490-RBM4-P0

Wilm's Tumor 1 General Information
Alternate Names
Wilms tumor protein WT1, Wilms tumor antigen, Wilms Tumor 1
Molecular Weight
47-55kDa
Chromosomal Location
11p13
Curated Database and Bioinformatic Data
Gene Symbol	WT1
Entrez Gene ID	7490
Ensemble Gene ID	ENSG00000184937
RefSeq Protein Accession(s)	NP_077742, NP_001185481, NP_077744, NP_000369, NP_001185480
RefSeq mRNA Accession(s)	NM_024426, NM_024425, NM_001198551, NM_001198552, NM_024424 NM_000378
RefSeq Genomic Accession(s)	NC_018922, NC_000011, NG_009272,
UniProt ID(s)	A0A1W2PQQ0, Q6PI38, P19544, A0A1W2PR07, A0A1W2PPP2
UniGene ID(s)	A0A1W2PQQ0, Q6PI38, P19544, A0A1W2PR07, A0A1W2PPP2
HGNC ID(s)	12796
Cosmic ID(s)	WT1
KEGG Gene ID(s)	hsa:7490
PharmGKB ID(s)	PA37395
General Description of Wilm's Tumor 1.
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Human Anti-Wilm's Tumor 1 Antibody Product Attributes

Species: Human

Tested Applications: Immunohistochemistry (IHC).

Clonality: Recombinant Monoclonal

Anti-Wilm's Tumor 1 Antibody Clone: WT1/1434R

Clone WT1/1434R Host and Isotype: Rabbit IgG kappa

Anti-Human Wilm's Tumor 1 Positive Control Sample: K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.

Cellular Localization of Antibody Nuclear & Cytoplasmic

Buffer and Stabilizer: 10mM PBS with or without 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant human full-length WT1 protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Wilm's Tumor 1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-WT1 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Wilm's Tumor 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, cells in the endometrial stroma in endometrium, glandular cells in the fallopian tube, cells in the glomeruli in kidney and cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in myoepithelial cells in the breast, cells in the endometrial stroma in endometrium, glandular cells in the fallopian tube, cells in the glomeruli in kidney and cells in the seminiferous ducts in testis. Low, but measureable presence of Wilm's Tumor 1 could be seen inglandular cells in the breast and epididymis, cells in the tubules in kidney, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate and seminal vesicle, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue and cells in the red pulp in spleen. We were unable to detect Wilm's Tumor 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Wilm's Tumor 1 expression as measured by anti-Wilm's Tumor 1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	-	+++	-	-	-	-	++	+++	-	-	-	-	-	-	-	-
WT1 Variability	++	+	+	+	+	++	+	+	+	+	++	++	+	+	++	+	+	+	+	++

Limitations and Warranty

enQuire Bio's Wilm's Tumor 1 Recombinant Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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