FFPE human mesothelioma sections stained with 100 ul anti-Wilms Tumor 1 (clone WT1/857) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
FFPE human mesothelioma sections stained with 100 ul anti-Wilms Tumor 1 (clone WT1/857) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded mouse kidney sections stained with 100 ul anti-Wilms Tumor 1 (clone WT1/857) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded rat kidney sections stained with 100 ul anti-Wilms Tumor 1 (clone WT1/857) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin-embedded Mouse Kidney stained with WT1 Monoclonal Antibody (WT1/857).

Anti-Wilm's Tumor 1 Antibody Clone WT1/857

$ 298.00
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SKU: 7490-MSM2
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7490-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7490-MSM2-P1
Wilm's Tumor 1 General Information
Alternate Names
Wilms tumor protein WT1, Wilms tumor antigen, Wilms Tumor 1
Molecular Weight
47-55kDa
Chromosomal Location
11p13
Curated Database and Bioinformatic Data
Gene SymbolWT1
Entrez Gene ID7490
Ensemble Gene IDENSG00000184937
RefSeq Protein Accession(s)NP_077742, NP_001185481, NP_077744, NP_000369, NP_001185480
RefSeq mRNA Accession(s)NM_024426, NM_024425, NM_001198551, NM_001198552, NM_024424 NM_000378
RefSeq Genomic Accession(s)NC_018922, NC_000011, NG_009272,
UniProt ID(s)A0A1W2PQQ0, Q6PI38, P19544, A0A1W2PR07, A0A1W2PPP2
UniGene ID(s)A0A1W2PQQ0, Q6PI38, P19544, A0A1W2PR07, A0A1W2PPP2
HGNC ID(s)12796
Cosmic ID(s)WT1
KEGG Gene ID(s)hsa:7490
PharmGKB ID(s)PA37395
General Description of Wilm's Tumor 1.
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1, also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic, familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney, the urogenital system. The WT1 gene is normally expressed in fetal kidney, mesothelium,, its expression has been suggested as a marker for Wilm s tumor, mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma,, desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Human, Mouse, and Rat Anti-Wilm's Tumor 1 Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Wilm's Tumor 1 Antibody Clone: WT1/857
Clone WT1/857 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat Wilm's Tumor 1 Positive Control Sample: K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human WT1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Wilm's Tumor 1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-WT1 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Wilm's Tumor 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, cells in the endometrial stroma in endometrium, glandular cells in the fallopian tube, cells in the glomeruli in kidney and cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in myoepithelial cells in the breast, cells in the endometrial stroma in endometrium, glandular cells in the fallopian tube, cells in the glomeruli in kidney and cells in the seminiferous ducts in testis. Low, but measureable presence of Wilm's Tumor 1 could be seen inglandular cells in the breast and epididymis, cells in the tubules in kidney, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate and seminal vesicle, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue and cells in the red pulp in spleen. We were unable to detect Wilm's Tumor 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Wilm's Tumor 1 expression as measured by anti-Wilm's Tumor 1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - +++ - - - - ++ +++ - - - - - - - -
WT1 Variability ++ + + + + ++ + + + + ++ ++ + + ++ + + + + ++

Limitations and Warranty

enQuire Bio's Wilm's Tumor 1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Wilm's Tumor 1 Antibody Clone WT1/857

https://enquirebio.com/wp-content/uploads/2019/01/Wilms Tumor 1 IHC Human Mesothelioma (WT1 857)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Wilms Tumor 1 IHC Mouse Kidney (WT1 857)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Wilms Tumor 1 IHC Rat kidney (WT1 857)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-7490-MSM2-P1-anti-Wilms-Tumor-1-antibody-300x225.jpeg