Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-ZAP70 (clone ZAP70/528 + 2F3.2) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.
Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-ZAP70 (clone ZAP70/528 + 2F3.2) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.

Anti-ZAP70 Antibody Clone ZAP70/528 + 2F3.2

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7535-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7535-MSM3-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 7535-MSM3-P1
ZAP70 General Information
Alternate Names
ZAP-70, Zeta-chain-associated protein kinase 70
Molecular Weight
70kDa
Chromosomal Location
2q12
Curated Database and Bioinformatic Data
Gene SymbolZAP70
Entrez Gene ID7535
Ensemble Gene IDENSG00000115085
RefSeq Protein Accession(s)XP_016860356, XP_016860357, XP_016860358, NP_997402, XP_016860359, NP_001070
RefSeq mRNA Accession(s)XM_017004868, XR_001738926, XR_001738927 NM_207519, XM_017004867, XR_001738925, NM_001079, XM_017004869, XM_017004870
RefSeq Genomic Accession(s)NC_018913, NC_000002, NG_007727
UniProt ID(s)P43403
UniGene ID(s)P43403
HGNC ID(s)12858
Cosmic ID(s)ZAP70
KEGG Gene ID(s)hsa:7535
PharmGKB ID(s)PA37447
General Description of ZAP70.
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells, natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative), Ig-unmutated (anti-ZAP70 positive) CLL subtypes, can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Human Anti-ZAP70 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-ZAP70 Antibody Clone: ZAP70/528 + 2F3.2
Clone ZAP70/528 + 2F3.2 Host and Isotype: Mouse IgG
Anti-Human ZAP70 Positive Control Sample: Jurkat cells. Tonsil or lymph node.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ZAP70 protein (ZAP70/528); Recombinant ZAP-70 protein including residues 1-254, encompassing SH2 domains of human ZAP70 (2F3.2)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

ZAP70 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in ZAP70 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of ZAP70 could be seen inmacrophages in lung and cells in the white pulp in spleen. We were unable to detect ZAP70 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of ZAP70 expression as measured by anti-ZAP70 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - - - - - - - - - - - - - - - - - -
ZAP70 Variability ++ + + + + + + + + ++ + + + + + ++ ++ + + +

Limitations and Warranty

enQuire Bio's ZAP70 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-ZAP70 Antibody Clone ZAP70/528 + 2F3.2”

Your email address will not be published.

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-ZAP70 Antibody Clone ZAP70/528 + 2F3.2

https://enquirebio.com/wp-content/uploads/2019/01/ZAP70 IHC human Tonsil (ZAP70 528 + 2F3.2)-300x225.jpg