Human Anti-ZAP70 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-ZAP70 Antibody Clone: ZAP70/528 + 2F3.2
Clone ZAP70/528 + 2F3.2 Host and Isotype: Mouse IgG
Anti-Human ZAP70 Positive Control Sample: Jurkat cells. Tonsil or lymph node.
Cellular Localization of Antibody
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ZAP70 protein (ZAP70/528); Recombinant ZAP-70 protein including residues 1-254, encompassing SH2 domains of human ZAP70 (2F3.2)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
ZAP70 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in ZAP70 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of ZAP70 could be seen inmacrophages in lung and cells in the white pulp in spleen. We were unable to detect ZAP70 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of ZAP70 expression as measured by anti-ZAP70 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's ZAP70 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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