Western Blot Analysis of THP-1 Cell and human Brain Tissue Lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).
Western Blot Analysis of THP-1 Cell and human Brain Tissue Lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).SDS-PAGE Analysis Purified ATG5 Mouse Monoclonal Antibody (ATG5/2101).Confirmation of Integrity and Purity of Antibody.Analysis of Protein Array containing more than 19,000 full-length human proteins using ATG5 Mouse Monoclonal Antibody (ATG5/2101). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).

ATG5 (Autophagy Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 9474-MSM1
Species: Human
Tested Applications: ELISA, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Size
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Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 9474-MSM1-P0
ATG5 (Autophagy Marker) General Information
Alternate Names
Autophagy protein 5 (APG5); APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5
Molecular Weight
50-32kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol9474
Entrez Gene IDATG5
UniProt ID(s)Q9H1Y0
UniGene ID(s)Hs486063
COSMIC ID Link(s)ATG5
KEGG Gene ID(s)hsa:9474
General Description of ATG5 (Autophagy Marker).
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Human Anti-ATG5 (Autophagy Marker) Antibody Product Attributes

Species: Human
Tested Applications: ELISA, WB, IHC.
Clonality: Monoclonal
Anti-ATG5 (Autophagy Marker) Antibody Clone: ATG5/2101
Clone ATG5/2101 Host and Isotype: Mouse IgG1, kappa
Anti-Human ATG5 (Autophagy Marker) Positive Control Sample: THP-1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum
Cellular Localization of Antibody ATG5/2101 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment of human ATG5 protein (apprx. aa 1-119) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

ATG5 (Autophagy Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, ATG5 (Autophagy Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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ATG5 (Autophagy Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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