Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-Beta Catenin (clone CTNNB1/2098) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Analysis of Protein Array containing more than 19,000 full-length human proteins using Catenin, beta (CTNNB1) Mouse Monoclonal Antibody (CTNBB1/2098). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Catenin, beta (CTNNB1) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 199.00$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1499-MSM11
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1499-MSM11-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
1499-MSM11-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
1499-MSM11-P1ABX Size: 100 ug, Tag: Unconjugated $ 429.00
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SKU: 1499-MSM11-P0
Catenin, beta (CTNNB1) General Information
Alternate Names
Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
Molecular Weight
92kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol1499
Entrez Gene IDCTNNB1
UniProt ID(s)P35222
UniGene ID(s)Hs476018
COSMIC ID Link(s)CTNNB1
KEGG Gene ID(s)hsa:1499
General Description of Catenin, beta (CTNNB1).
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Human Anti-Catenin, beta (CTNNB1) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-Catenin, beta (CTNNB1) Antibody Clone: CTNNB1/2098
Clone CTNNB1/2098 Host and Isotype: Mouse IgG2b, kappa
Anti-Human Catenin, beta (CTNNB1) Positive Control Sample: HeLa or MCF-7 cells. Breast carcinoma
Cellular Localization of Antibody CTNNB1/2098 Staining: Cell surface and cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human beta-Catenin (CTNNB1) protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Catenin, beta (CTNNB1) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CTNNB1 antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Beta Catenin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Beta Catenin could be seen in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex, fibroblasts in skin, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary and Purkinje cells in the cerebellum. We were unable to detect Beta Catenin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Beta Catenin expression as measured by anti-Beta Catenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ +++ + +++ +++ +++ - +++ +++ +++ +++ ++ +++ ++ + +++ +++
CTNNB1 Variability ++ ++ ++ + + +++ ++ ++ ++ + + ++ + + ++ + ++ ++ + ++

Limitations and Warranty

enQuire Bio's product, Catenin, beta (CTNNB1) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Catenin, beta (CTNNB1) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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