FFPE human spleen sections stained with 100 ul anti-CD3 (clone C3e/2478) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Data from SDS-PAGE analysis of Anti-CD3 antibody (Clone C3e/2478). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD3e Mouse Monoclonal Antibody (C3e/2478) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

CD3e (T-Cell Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: CD3e (T-Cell Marker) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 916-MSM14-P0
Availability: InStock

$ 199.00 – $ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 916-MSM14
Species: Human, Chimpanzee
Tested Applications: IHC, ELISA
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
916-MSM14-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
916-MSM14-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
916-MSM14-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 429.00

Shipping Information

In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 916-MSM14-P0

CD3e (T-Cell Marker) General Information
Alternate Names
CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
Molecular Weight
20kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	916
Entrez Gene ID	CD3E
UniProt ID(s)	P07766
UniGene ID(s)	Hs3003
COSMIC ID Link(s)	CD3E
KEGG Gene ID(s)	hsa:916
General Description of CD3e (T-Cell Marker).
Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Human, Chimpanzee Anti-CD3e (T-Cell Marker) Antibody Product Attributes

Species: Human, Chimpanzee

Tested Applications: IHC, ELISA.

Clonality: Monoclonal

Anti-CD3e (T-Cell Marker) Antibody Clone: C3e/2478

Clone C3e/2478 Host and Isotype: Mouse IgG1, kappa

Anti-Human, Chimpanzee CD3e (T-Cell Marker) Positive Control Sample: Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Cellular Localization of Antibody C3e/2478 Staining: Cell Surface and Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant human CD3e fragment (apprx. aa 23-119) (Please call for additional information.)

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

CD3e (T-Cell Marker) Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3e antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3e could be seen in. We were unable to detect CD3e in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's product, CD3e (T-Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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CD3e (T-Cell Marker) Ultraspecific Antibody Tested against >20,000 Human Proteins