Immunohistochemistry data from FFPE human tonsil stained with CD40 monoclonal antibody clone C40/2383.
Immunohistochemistry data from FFPE human tonsil stained with CD40 monoclonal antibody clone C40/2383.Analysis of Protein Array containing more than 19,000 full-length human proteins using CD40 Mouse Recombinant Monoclonal Antibody (C40/2383) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.SDS-PAGE Analysis Purified CD40 Mouse Monoclonal Antibody (C40/2383).Confirmation of Integrity and Purity of Antibody.

Human CD40 Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 958-MSM3
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: 958-MSM3-P0
CD40 / TNFRSF5 / CD40L-Receptor General Information
Alternate Names
B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; GP39; HIGM1; IGM; IMD3; p50; TBAM; TNF receptor superfamily member 5; TNFRSF5; TRAP
Molecular Weight
43kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol 958
Entrez Gene ID CD40
UniProt ID(s) P25942
UniGene ID(s) Hs472860
COSMIC ID Link(s) CD40
KEGG Gene ID(s) hsa:958
General Description of CD40 / TNFRSF5 / CD40L-Receptor.
CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Human Anti-CD40 / TNFRSF5 Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-CD40 / TNFRSF5 / CD40L-Receptor Antibody Clone: C40/2383
Clone C40/2383 Host and Isotype: Mouse IgG2b, kappa
Anti-Human CD40 / TNFRSF5 / CD40L-Receptor Positive Control Sample: L-428 Cells. Tonsil, Spleen or Thymus
Cellular Localization of Antibody C40/2383 Staining: Cell Surface and Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment of human CD40 protein (apprx. aa 29-107) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

CD40 / TNFRSF5 / CD40L-Receptor Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD40 / TNFRSF5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD40 / TNFRSF5 could be seen inlymphoid tissue in appendix and macrophages in lung. We were unable to detect CD40 / TNFRSF5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD40 / TNFRSF5 expression as measured by anti-CD40 / TNFRSF5 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - + - - - - - - - - + -
CD40 Variability + + + + + + + + ++ ++ + ++ + + + + + + ++ +

Limitations and Warranty

enQuire Bio's product, CD40 / TNFRSF5 / CD40L-Receptor MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Human CD40 Ultraspecific Antibody

Tested against >20,000 Human Proteins

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