Analysis of Protein Array containing >19,000 full-length human proteins using CD71 Mouse Monoclonal Antibody (TFRC/1839) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing >19,000 full-length human proteins using CD71 Mouse Monoclonal Antibody (TFRC/1839) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) MonoSpecific Antibody

$ 199.00$ 429.00
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SKU: 7037-MSM9
Species: Human
Tested Applications: ELISA, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 7037-MSM9-P0
CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) General Information
Alternate Names
Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR
Molecular Weight
85-95kDa (monomer); 190kDa (dimer)
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol7037
Entrez Gene IDTFRC
UniProt ID(s)P02786
UniGene ID(s)Hs529618
COSMIC ID Link(s)TFRC
KEGG Gene ID(s)hsa:7037
General Description of CD71 / Transferrin Receptor (TFRC) (Extracellular Domain).
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Human Anti-CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody Product Attributes

Species: Human
Tested Applications: ELISA, WB, IHC.
Clonality: Monoclonal
Anti-CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody Clone: TFRC/1839
Clone TFRC/1839 Host and Isotype: Mouse IgG2b, kappa
Anti-Human CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Positive Control Sample: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.
Cellular Localization of Antibody TFRC/1839 Staining: Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human extracellular TFRC protein fragment (apprx. aa 94-212) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TFRC antibody staining is expected to be primarily localized to the endosomes and lysosomes.

Observed Antibody Staining Data By Tissue Type:

Variations in CD71 / Transferrin Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow and trophoblastic cells in the placenta. Low, but measureable presence of CD71 / Transferrin Receptor could be seen inlymphoid tissue in appendix, glandular cells in the breast, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, neuronal cells in the cerebral cortex, squamous epithelial cells in the cervix, uterine, glandular cells in the endometrium, epididymis and gallbladder, myocytes in heart muscle, hepatocytes in liver, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the parathyroid gland and prostate, keratinocytes in skin, melanocytes in skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle, peripheral nerve in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis and non-germinal center cells in the tonsil. We were unable to detect CD71 / Transferrin Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD71 / Transferrin Receptor expression as measured by anti-CD71 / Transferrin Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - ++ ++ ++ ++ +++ + + + + ++ + - - ++ - - - ++
TFRC Variability ++ + ++ + ++ ++ + ++ +++ ++ ++ ++ ++ + ++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's product, CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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