FFPE human placent sections stained with 100 ul anti-DNMT3A (clone PCRP-DNMT3A-1E2) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.Data from SDS-PAGE analysis of Anti-DNMT3A antibody (Clone PCRP-DNMT3A-1E2). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

DNMT3A / DNA Methyltransferase 3 Alpha Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1788-MSM1
Species: Human
Tested Applications: IP, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1788-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
1788-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
1788-MSM1-P1ABX Size: 100 ug, Tag: Unconjugated $ 488.00
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SKU: 1788-MSM1-P0
DNMT3A / DNA Methyltransferase 3 Alpha General Information
Alternate Names
DNA methyltransferase 3 alpha; DNA methyltransferase HsaIIIA; DNAMTaseHsaIIIA; DNMT; DNMT3A2; M.HsaIIIA; MCMT; TBRS
Molecular Weight
102kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol1788
Entrez Gene IDDNMT3A
UniProt ID(s)Q16655
UniGene ID(s)Hs154069
COSMIC ID Link(s)DNMT3A
KEGG Gene ID(s)hsa:1788
General Description of DNMT3A / DNA Methyltransferase 3 Alpha.
The specificity of this monoclonal antibody to its intended target was validated by HuProtTMArray, containing more than 19,000, full-length human proteins.DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Sadenosyl-L-methionin

Human Anti-DNMT3A / DNA Methyltransferase 3 Alpha Antibody Product Attributes

Species: Human
Tested Applications: IP, WB, IHC.
Clonality: Monoclonal
Anti-DNMT3A / DNA Methyltransferase 3 Alpha Antibody Clone: PCRP-DNMT3A-1E2
Clone PCRP-DNMT3A-1E2 Host and Isotype: Mouse IgG1
Anti-Human DNMT3A / DNA Methyltransferase 3 Alpha Positive Control Sample: HeLa cells orPlacenta
Cellular Localization of Antibody PCRP-DNMT3A-1E2 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human DNMT3A protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

DNMT3A / DNA Methyltransferase 3 Alpha Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, DNMT3A / DNA Methyltransferase 3 Alpha MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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DNMT3A / DNA Methyltransferase 3 Alpha Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-DNMT3A Specificity Assay (Array)-300x121.jpghttps://enquirebio.com/wp-content/uploads/2019/01/DNMT3A IHC human Placent (PCRP-DNMT3A-1E2)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-DNMT3A(PCRP-DNMT3A-1E2) Gel-217x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-1788-MSM1-P0-anti-DNMT3A-DNA-Methyltransferase-3-Alpha-antibody-1-300x121.jpeg