Data from SDS-PAGE analysis of Anti-E-Cadherin antibody (Clone rCDH1/1525). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.

SDS-PAGE Analysis Purified E-Cadherin Mouse Recombinant Monoclonal Ab (rCDH1/1525).Confirmation of Purity and Integrity of Antibody.

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 999-MSM7-P0
Availability: InStock

$ 199.00 – $ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 999-MSM7
Species: Human. Does not react with Mouse and Rat.
Tested Applications: WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
999-MSM7-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
999-MSM7-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
999-MSM7-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 429.00

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In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 999-MSM7-P0

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) General Information
Alternate Names
Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)
Molecular Weight
120-80kDa (Mature); 135kDa (Precursor)
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	999
Entrez Gene ID	CDH1
UniProt ID(s)	P12830
UniGene ID(s)	Hs461086
COSMIC ID Link(s)	CDH1
KEGG Gene ID(s)	hsa:999
General Description of E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker).
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as Î²-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Human. Does not react with Mouse and Rat. Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody Product Attributes

Species: Human. Does not react with Mouse and Rat.

Tested Applications: WB, IHC.

Clonality: Monoclonal

Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody Clone: rCDH1/1525

Clone rCDH1/1525 Host and Isotype: Mouse IgG1, kappa

Anti-Human. Does not react with Mouse and Rat. E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Positive Control Sample: MCF-7, LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.

Cellular Localization of Antibody rCDH1/1525 Staining: Cell Surface

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant full-length human E-Cadherin protein

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDH1 antibody staining is expected to be primarily localized to the cell junctions, golgi apparatus and plasma membrane. There is variability in either the signal strength or the localization of signal in plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in E-Cadherin / CD324 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of E-Cadherin / CD324 could be seen inmacrophages in lung. We were unable to detect E-Cadherin / CD324 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of E-Cadherin / CD324 expression as measured by anti-E-Cadherin / CD324 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	+++	++	+++	+++	+++	-	++	++	++	-	++	+++	+++	+++	-	++	+++	++	+++	+++
CDH1 Variability	++	++	++	+	+	+	++	++	++	+	++	++	+	+	++	++	++	++	+	++

Limitations and Warranty

enQuire Bio's product, E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Ultraspecific Antibody Tested against >20,000 Human Proteins