SDS-PAGE Analysis Purified E-Cadherin Mouse Recombinant Monoclonal Ab (rCDH1/1525).Confirmation of Purity and Integrity of Antibody.
SDS-PAGE Analysis Purified E-Cadherin Mouse Recombinant Monoclonal Ab (rCDH1/1525).Confirmation of Purity and Integrity of Antibody.Analysis of Protein Array containing >19,000 full-length human proteins using E-Cadherin Mouse Monoclonal Antibody (rCDH1/1525) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Western Blot Analysis of MCF-7 Cell Lysate using E-Cadherin Mouse Recombinant Monoclonal Antibody (rCDH1/1525).

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 999-MSM7
Species: Human. Does not react with Mouse and Rat.
Tested Applications: WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: 999-MSM7-P0
E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) General Information
Alternate Names
Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)
Molecular Weight
120-80kDa (Mature); 135kDa (Precursor)
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol999
Entrez Gene IDCDH1
UniProt ID(s)P12830
UniGene ID(s)Hs461086
COSMIC ID Link(s)CDH1
KEGG Gene ID(s)hsa:999
General Description of E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker).
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Human. Does not react with Mouse and Rat. Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody Product Attributes

Species: Human. Does not react with Mouse and Rat.
Tested Applications: WB, IHC.
Clonality: Monoclonal
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody Clone: rCDH1/1525
Clone rCDH1/1525 Host and Isotype: Mouse IgG1, kappa
Anti-Human. Does not react with Mouse and Rat. E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Positive Control Sample: MCF-7, LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Cellular Localization of Antibody rCDH1/1525 Staining: Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human E-Cadherin protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDH1 antibody staining is expected to be primarily localized to the cell junctions, golgi apparatus and plasma membrane. There is variability in either the signal strength or the localization of signal in plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in E-Cadherin / CD324 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of E-Cadherin / CD324 could be seen inmacrophages in lung. We were unable to detect E-Cadherin / CD324 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of E-Cadherin / CD324 expression as measured by anti-E-Cadherin / CD324 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ +++ - ++ ++ ++ - ++ +++ +++ +++ - ++ +++ ++ +++ +++
CDH1 Variability ++ ++ ++ + + + ++ ++ ++ + ++ ++ + + ++ ++ ++ ++ + ++

Limitations and Warranty

enQuire Bio's product, E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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