SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).Confirmation of Integrity and Purity of Antibody.
SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).Confirmation of Integrity and Purity of Antibody.Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).

Estrogen Receptor, alpha (Marker of Estrogen Dependence) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 199.00$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2099-MSM15
Species: Human
Tested Applications: WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2099-MSM15-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
2099-MSM15-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
2099-MSM15-P1ABX Size: 100 ug, Tag: Unconjugated $ 429.00
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SKU: 2099-MSM15-P0
Estrogen Receptor, alpha (Marker of Estrogen Dependence) General Information
Alternate Names
Estrogen Receptor alpha delta 4*5,6,7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1
Molecular Weight
~67kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol2099
Entrez Gene IDESR1
UniProt ID(s)P03372
UniGene ID(s)Hs208124
COSMIC ID Link(s)ESR1
KEGG Gene ID(s)hsa:2099
General Description of Estrogen Receptor, alpha (Marker of Estrogen Dependence).
This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Human Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody Product Attributes

Species: Human
Tested Applications: WB, IHC.
Clonality: Monoclonal
Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody Clone: ESR1/1935
Clone ESR1/1935 Host and Isotype: Mouse IgG2a, kappa
Anti-Human Estrogen Receptor, alpha (Marker of Estrogen Dependence) Positive Control Sample: MCF-7 Cells. Breast or Endometrial Carcinoma.
Cellular Localization of Antibody ESR1/1935 Staining: Nucleus
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human Estrogen Receptor alpha protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Estrogen Receptor, alpha (Marker of Estrogen Dependence) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ESR1 antibody staining is expected to be primarily localized to the nucleus and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Estrogen Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. Low, but measureable presence of Estrogen Receptor could be seen insmooth muscle cells in the smooth muscle. We were unable to detect Estrogen Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Estrogen Receptor expression as measured by anti-Estrogen Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ - - - + - - - - - - - - - - - - - - -
ESR1 Variability ++ ++ + + ++ + + + + + + ++ + + + + + + + +

Limitations and Warranty

enQuire Bio's product, Estrogen Receptor, alpha (Marker of Estrogen Dependence) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Estrogen Receptor, alpha (Marker of Estrogen Dependence) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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