SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1935).

Estrogen Receptor, alpha (Marker of Estrogen Dependence) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: Estrogen Receptor, alpha (Marker of Estrogen Dependence) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 2099-MSM15-P0
Availability: InStock

$ 199.00 – $ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 2099-MSM15
Species: Human
Tested Applications: WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a, kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
2099-MSM15-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
2099-MSM15-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
2099-MSM15-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 429.00

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In Stock.

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 2099-MSM15-P0

Estrogen Receptor, alpha (Marker of Estrogen Dependence) General Information
Alternate Names
Estrogen Receptor alpha delta 45,6,7/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1
Molecular Weight
~67kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	2099
Entrez Gene ID	ESR1
UniProt ID(s)	P03372
UniGene ID(s)	Hs208124
COSMIC ID Link(s)	ESR1
KEGG Gene ID(s)	hsa:2099
General Description of Estrogen Receptor, alpha (Marker of Estrogen Dependence).
This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Human Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody Product Attributes

Species: Human

Tested Applications: WB, IHC.

Clonality: Monoclonal

Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody Clone: ESR1/1935

Clone ESR1/1935 Host and Isotype: Mouse IgG2a, kappa

Anti-Human Estrogen Receptor, alpha (Marker of Estrogen Dependence) Positive Control Sample: MCF-7 Cells. Breast or Endometrial Carcinoma.

Cellular Localization of Antibody ESR1/1935 Staining: Nucleus

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant full-length human Estrogen Receptor alpha protein

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Estrogen Receptor, alpha (Marker of Estrogen Dependence) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ESR1 antibody staining is expected to be primarily localized to the nucleus and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Estrogen Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, glandular cells in the cervix, uterine, endometrium and fallopian tube, squamous epithelial cells in the cervix and uterine. Low, but measureable presence of Estrogen Receptor could be seen insmooth muscle cells in the smooth muscle. We were unable to detect Estrogen Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Estrogen Receptor expression as measured by anti-Estrogen Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	+++	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
ESR1 Variability	++	++	+	+	++	+	+	+	+	+	+	++	+	+	+	+	+	+	+	+

Limitations and Warranty

enQuire Bio's product, Estrogen Receptor, alpha (Marker of Estrogen Dependence) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Estrogen Receptor, alpha (Marker of Estrogen Dependence) Ultraspecific Antibody Tested against >20,000 Human Proteins