Anti-HER2 Specificity Assay (Array)
Data from SDS-PAGE analysis of Anti-HER2 antibody (Clone ERBB2/2453). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2453). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

HER-2 / c-erbB-2 / neu / CD340 Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 158.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2064-MSM13
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2064-MSM13-P0 Size: 20 ug, Tag: Unconjugated $ 158.00
2064-MSM13-P1 Size: 100 ug, Tag: Unconjugated $ 348.00
2064-MSM13-P1ABX Size: 100 ug, Tag: Unconjugated $ 348.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 2064-MSM13-P0
HER-2 / c-erbB-2 / neu / CD340 General Information
Alternate Names
p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
Molecular Weight
185kDa
Chromosomal Location
17q12
Curated Database and Bioinformatic Data
Gene Symbol2064
Entrez Gene IDERBB2
UniProt ID(s)IDP04626
UniGene ID(s)Hs446352
COSMIC ID Link(s)ERBB2
KEGG Gene ID(s)hsa:2064
General Description of HER-2 / c-erbB-2 / neu / CD340.
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Human Anti-HER-2 / c-erbB-2 / neu / CD340 Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-HER-2 / c-erbB-2 / neu / CD340 Antibody Clone: ERBB2/2453
Clone ERBB2/2453 Host and Isotype: Mouse IgG2a, kappa
Anti-Human HER-2 / c-erbB-2 / neu / CD340 Positive Control Sample: SKBR-3 Cells or Breast Cancers.
Cellular Localization of Antibody ERBB2/2453 Staining: Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human HER-2 protein fragment (apprx. aa 311-462) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

HER-2 / c-erbB-2 / neu / CD340 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ERBB2 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in HER2 / ErbB2 / neu / CD340 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of HER2 / ErbB2 / neu / CD340 could be seen inglandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine and thyroid gland, hematopoietic cells in the bone marrow, hepatocytes in liver, melanocytes in skin, pneumocytes in lung, squamous epithelial cells in the cervix, uterine, esophagus, tonsil and vagina and glandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle and small intestine and thyroid gland. We were unable to detect HER2 / ErbB2 / neu / CD340 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of HER2 / ErbB2 / neu / CD340 expression as measured by anti-HER2 / ErbB2 / neu / CD340 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - - - - - - + - - - - - - - - - - - +
ERBB2 Variability +++ + + ++ ++ + ++ ++ + + + ++ ++ + + ++ + ++ + ++

Limitations and Warranty

enQuire Bio's product, HER-2 / c-erbB-2 / neu / CD340 MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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HER-2 / c-erbB-2 / neu / CD340 Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-HER2 Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/HER2 IHC Human Breast Carcinoma (ERBB2 2453)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-HER2(ERBB2 2453) SDS-PAGE-229x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-2064-MSM13-P0-anti-HER-2-c-erbB-2-neu-CD340-antibody-1-300x120.jpeg