Analysis of Protein Array containing >19,000 full-length human proteins usingPgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing >19,000 full-length human proteins usingPgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.SDS-PAGE Analysis of Purified Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775

PGP9.5 / UchL1 (pan-Neuronal Marker) MonoSpecific Antibody

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SKU: 7345-MSM5
Species: Human and Rat. Shows a broad species reactivity.
Tested Applications: WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 7345-MSM5-P0
PGP9.5 / UchL1 (pan-Neuronal Marker) General Information
Alternate Names
Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1
Molecular Weight
20-30kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol7345
Entrez Gene IDUCHL1
UniProt ID(s)P09936
UniGene ID(s)Hs518731
COSMIC ID Link(s)UCHL1
KEGG Gene ID(s)hsa:7345
General Description of PGP9.5 / UchL1 (pan-Neuronal Marker).
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Human and Rat. Shows a broad species reactivity. Anti-PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody Product Attributes

Species: Human and Rat. Shows a broad species reactivity.
Tested Applications: WB, IHC.
Clonality: Monoclonal
Anti-PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody Clone: rUCHL1/775
Clone rUCHL1/775 Host and Isotype: Mouse IgG1, kappa
Anti-Human and Rat. Shows a broad species reactivity. PGP9.5 / UchL1 (pan-Neuronal Marker) Positive Control Sample: Cerebellum
Cellular Localization of Antibody rUCHL1/775 Staining: Cytoplasmic. Endoplasmic Reticulum membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human UCHL1 protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

PGP9.5 / UchL1 (pan-Neuronal Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-UCHL1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in PGP9.5 / UchL1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, peripheral nerve/ganglion in colon, neuronal cells in the hippocampus, cells in the tubules in kidney and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, peripheral nerve/ganglion in colon, neuronal cells in the hippocampus, cells in the tubules in kidney and islets of Langerhans in pancreas. Low, but measureable presence of PGP9.5 / UchL1 could be seen in cells in the seminiferous ducts in testis and Leydig cells in the testis. We were unable to detect PGP9.5 / UchL1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of PGP9.5 / UchL1 expression as measured by anti-PGP9.5 / UchL1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + - - - + - - - - - - - - - - - - - -
UCHL1 Variability + +++ + + + ++ + + ++ + + + + + + + + ++ + +

Limitations and Warranty

enQuire Bio's product, PGP9.5 / UchL1 (pan-Neuronal Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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