SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using Podocalyxin Mouse Monoclonal Antibody (PODXL/2185).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Endometrium stained with Podocalyxin Mouse Monoclonal Antibody (PODXL/2185).

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 5420-MSM5-P0
Availability: InStock

$ 199.00 – $ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 5420-MSM5
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
5420-MSM5-P0	Size: 20 ug, Tag: Unconjugated	$ 228.00
5420-MSM5-P1	Size: 100 ug, Tag: Unconjugated	$ 488.00
5420-MSM5-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 488.00

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In Stock.

Flat Rate Shipping Anywhere in the US: $45

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Certificate of Analysis and Tags (Coming Soon)

SKU: 5420-MSM5-P0

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) General Information
Alternate Names
PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1
Molecular Weight
165-170kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	5420
Entrez Gene ID	PODXL
UniProt ID(s)	O00592
UniGene ID(s)	Hs732423
COSMIC ID Link(s)	PODXL
KEGG Gene ID(s)	hsa:5420
General Description of Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker).
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Human Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody Product Attributes

Species: Human

Tested Applications: IHC.

Clonality: Monoclonal

Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody Clone: PODXL/2185

Clone PODXL/2185 Host and Isotype: Mouse IgG1

Anti-Human Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Positive Control Sample: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Cellular Localization of Antibody PODXL/2185 Staining: Cell Surface and Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant fragment of human Podocalyxin (apprx. aa 310-447) (Please call for additional information.)

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-PODXL antibody staining is expected to be primarily localized to the plasma membrane, microtubule organizing center and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Podocalyxin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. More moderate antibody staining intensity was present in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. Low, but measureable presence of Podocalyxin could be seen inglandular cells in the breast, decidual cells in the placenta and glandular cells in the seminal vesicle. We were unable to detect Podocalyxin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Podocalyxin expression as measured by anti-Podocalyxin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	++	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
PODXL Variability	+	+	+	+	+++	++	++	+	+	+	+	++	++	+	+	+	+	++	+	+

Limitations and Warranty

enQuire Bio's product, Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Ultraspecific Antibody Tested against >20,000 Human Proteins