Human Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody Product Attributes
Tested Applications: IHC.
Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody Clone: PODXL/2185
Clone PODXL/2185 Host and Isotype: Mouse IgG1
Anti-Human Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Positive Control Sample: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney
Cellular Localization of Antibody PODXL/2185 Staining: Cell Surface and Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment of human Podocalyxin (apprx. aa 310-447) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.
Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-PODXL antibody staining is expected to be primarily localized to the plasma membrane, microtubule organizing center and vesicles.
Observed Antibody Staining Data By Tissue Type:Variations in Podocalyxin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. More moderate antibody staining intensity was present in endothelial cells in the cerebral cortex, glandular cells in the fallopian tube and cells in the glomeruli in kidney. Low, but measureable presence of Podocalyxin could be seen inglandular cells in the breast, decidual cells in the placenta and glandular cells in the seminal vesicle. We were unable to detect Podocalyxin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Podocalyxin expression as measured by anti-Podocalyxin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's product, Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.
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