Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778).Confirmation of Integrity and Purity of Antibody.

Spectrin beta III (SPTBN2) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 159.00$ 349.00
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SKU: 6712-MSM5
Species: Human
Tested Applications: IHC, ELISA
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 6712-MSM5-P0
Spectrin beta III (SPTBN2) General Information
Alternate Names
Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2
Molecular Weight
246kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol6712
Entrez Gene IDSPTBN2
UniProt ID(s)O15020
UniGene ID(s)Hs26915
COSMIC ID Link(s)SPTBN2
KEGG Gene ID(s)hsa:6712
General Description of Spectrin beta III (SPTBN2).
Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin α and spectrin β are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β I (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin β III is highly homologous to both spectrin β I and spectrin β II. Spectrin β III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Human Anti-Spectrin beta III (SPTBN2) Antibody Product Attributes

Species: Human
Tested Applications: IHC, ELISA.
Clonality: Monoclonal
Anti-Spectrin beta III (SPTBN2) Antibody Clone: SPTBN2/1778
Clone SPTBN2/1778 Host and Isotype: Mouse IgG1, kappa
Anti-Human Spectrin beta III (SPTBN2) Positive Control Sample: HeLa cells. Pancreas or Liver.
Cellular Localization of Antibody SPTBN2/1778 Staining: Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment of human Spectrin beta III (SPTBN2) protein (apprx. aa 356-475) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Spectrin beta III (SPTBN2) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-SPTBN2 antibody staining is expected to be primarily localized to the cell junctions and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Spectrin beta III antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. Low, but measureable presence of Spectrin beta III could be seen inglandular cells in the breast, respiratory epithelial cells in the bronchus, glandular cells in the endometrium, epididymis and gallbladder, hepatocytes in liver, macrophages in lung, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, Langerhans in skin, cells in the red pulp in spleen and cells in the seminiferous ducts in testis. We were unable to detect Spectrin beta III in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Spectrin beta III expression as measured by anti-Spectrin beta III antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + ++ ++ ++ ++ + + + + - - ++ + +++ - ++ + - +++ +
SPTBN2 Variability +++ ++ ++ + ++ +++ ++ +++ ++ + ++ +++ ++ + ++ ++ ++ ++ ++ +++

Limitations and Warranty

enQuire Bio's product, Spectrin beta III (SPTBN2) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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Spectrin beta III (SPTBN2) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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