Flow Cytometry of human Vimentin on Jurkat Cells.Black: Cells alone; Grey: Isotype Control.Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).
Flow Cytometry of human Vimentin on Jurkat Cells.Black: Cells alone; Grey: Isotype Control.Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452).Confirmation of Integrity and Purity of Antibody.Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452).

Vimentin (Mesenchymal Cell Marker) MonoSpecific Antibody

$ 199.00$ 429.00
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SKU: 7431-MSM1
Species: Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat.
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 7431-MSM1-P0
Vimentin (Mesenchymal Cell Marker) General Information
Alternate Names
VIM
Molecular Weight
57-60kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol7431
Entrez Gene IDVIM
UniProt ID(s)P08670
UniGene ID(s)Hs455493
COSMIC ID Link(s)VIM
KEGG Gene ID(s)hsa:7431
General Description of Vimentin (Mesenchymal Cell Marker).
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat. Anti-Vimentin (Mesenchymal Cell Marker) Antibody Product Attributes

Species: Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat.
Tested Applications: Flow, IF, WB, IHC.
Clonality: Monoclonal
Anti-Vimentin (Mesenchymal Cell Marker) Antibody Clone: VM452
Clone VM452 Host and Isotype: Mouse IgG1
Anti-Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat. Vimentin (Mesenchymal Cell Marker) Positive Control Sample: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Cellular Localization of Antibody VM452 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human vimentin protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Vimentin (Mesenchymal Cell Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Vimentin Anti-Human antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of Vimentin Anti-Human could be seen inglandular cells in the adrenal gland, respiratory epithelial cells in the bronchus, glial cells in the cerebral cortex, cells in the endometrial stroma in endometrium, glial cells in the hippocampus, bile duct cells in the liver, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, decidual cells in the placenta and squamous epithelial cells in the vagina. We were unable to detect Vimentin Anti-Human in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Vimentin Anti-Human expression as measured by anti-Vimentin Anti-Human antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + - ++ - ++ ++ ++ +++ +++ + ++ - +++ ++ ++ ++ +++ ++
VIM Variability + + ++ ++ ++ ++ ++ ++ + ++ + ++ + ++ + ++ + ++ + ++

Limitations and Warranty

enQuire Bio's product, Vimentin (Mesenchymal Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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