Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-ZAP70 (clone ZAP70/2035) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Data from SDS-PAGE analysis of Anti-ZAP70 antibody (Clone ZAP70/2035). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.

Analysis of Protein Array containing more than 19,000 full-length human proteins using ZAP70 Mouse Monoclonal Antibody (ZAP70/2035).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

ZAP70 (Chronic Lymphocytic Leukemia Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: ZAP70 (Chronic Lymphocytic Leukemia Marker) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 7535-MSM5-P0
Availability: InStock

$ 199.00 – $ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 7535-MSM5
Species: Human
Tested Applications: IHC, ELISA
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
7535-MSM5-P0	Size: 20 ug, Tag: Unconjugated	$ 199.00
7535-MSM5-P1	Size: 100 ug, Tag: Unconjugated	$ 429.00
7535-MSM5-P1ABX	Size: 100 ug, Tag: Unconjugated	$ 429.00

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In Stock.

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 7535-MSM5-P0

ZAP70 (Chronic Lymphocytic Leukemia Marker) General Information
Alternate Names
Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70;TZK; Zeta chain associated protein kinase 70kDa
Molecular Weight
70kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	7535
Entrez Gene ID	ZAP70
UniProt ID(s)	P43403
UniGene ID(s)	Hs234569
COSMIC ID Link(s)	ZAP70
KEGG Gene ID(s)	hsa:7535
General Description of ZAP70 (Chronic Lymphocytic Leukemia Marker).
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Human Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody Product Attributes

Species: Human

Tested Applications: IHC, ELISA.

Clonality: Monoclonal

Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody Clone: ZAP70/2035

Clone ZAP70/2035 Host and Isotype: Mouse IgG1, kappa

Anti-Human ZAP70 (Chronic Lymphocytic Leukemia Marker) Positive Control Sample: Jurkat cells. Tonsil or lymph node.

Cellular Localization of Antibody ZAP70/2035 Staining: Cell Surface and Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Recombinant fragment of human ZAP70 protein (apprx. aa 247-382) (Please call for additional information.)

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

ZAP70 (Chronic Lymphocytic Leukemia Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in ZAP70 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of ZAP70 could be seen inmacrophages in lung and cells in the white pulp in spleen. We were unable to detect ZAP70 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of ZAP70 expression as measured by anti-ZAP70 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
ZAP70 Variability	++	+	+	+	+	+	+	+	+	++	+	+	+	+	+	++	++	+	+	+

Limitations and Warranty

enQuire Bio's product, ZAP70 (Chronic Lymphocytic Leukemia Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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ZAP70 (Chronic Lymphocytic Leukemia Marker) Ultraspecific Antibody Tested against >20,000 Human Proteins