Human Erythropoietin / EPO Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Erythropoietin / EPO based upon sequence from: Human
Host: QP10347 protein expressed in Chinese Hamster Ovary Cells (CHO)
Tag: Though we don't consider it a tag, it is bound to the Human IgG1 Fc chain, in dimer form. See protein construction.
Protein Construction: This protein is a dimer of the EPO protein, bound to human IgG1 Fc, and produced in Chinese hamster ovary (CHO) cells. It is a glycosilated, polypeptide chain and the Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1.
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for application specific information for QP10347.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 determined by a cell proliferation assay using human megakaryoblastic leukemia cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
Full Length?Â Full-length less pro-sequence.Â Given the dimer, length and glycosilation, this protein migrates about 140kD.
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Concentration of Human Erythropoietin / EPO Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2um filtered sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid), lyophilized
Storage Conditions: Store at -20C to -80C.
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.