Anti-CD11b Antibody [ICRF44]

Human Anti-CD11b Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD11b Antibody Clone: ICRF44
Clone ICRF44 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11b Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11b antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD11b could be seen in cells in the molecular layer in cerebellum, glial cells in the caudate nucleus, cerebral cortex and hippocampus and macrophages in lung. We were unable to detect CD11b in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, and Cross AS. 2011. J. Leukoc. Biol. 90:313-321. (Immunoprecipitation)Chang WLW and Barry PA. 2010. Proc. Natl. Acad. Sci. 107:22647-2652. (Flow cytometry – Rhesus macaque)Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, and Kettritz R. 2010. J. Biol. Chem. 286:7070-7081. (in vitro blocking)Moreau A, Hill M, Thebault P, Deschamps JY, Chiffoleau E, Chauveau C, Moullier P, Anegon I, Alliot-Licht B, and Cuturi MC. 2009. FASEB J. 23:3070-3077. (Flow cytometry – cynomolgus macaque)Sengoku K, Takuma N, Miyamoto T, Horikawa M, and Ishikawa M. 2004. Hum. Reprod. 19: 639-644. (Immunofluorescence microscopy)David A, Kacher Y, Specks U, and Aviram I. 2003. J. Leukoc. Biol. 74:551-557. (Western blot)Rezzonico R, Imbert V, Chicheportiche R, and Dayer J-M. 2001. Blood. 97: 2932-2940. (in vitro activation)

Limitations and Warranty

enQuire Bio’s Human Anti-CD11b Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD3 Antibody [OKT3]

Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG2a isotype control.

Human Anti-CD3 Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: OKT3
Clone OKT3 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD3 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3 could be seen in. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Haga-Friedman A, Horovitz-Fried M, and Cohen CJ. Jun 2012. J. Immunol. 188:5538-5546. (in vitro activation)Bikker A, Moret FM, Kruize AA, Bijlsma JWJ, Lafeber FPJG, and van Roon JAG. Jun 2012. Rheumatology 51:996-1005 (in vitro activation)Bagnara D, Kaufman MS, Calissano C, et al. 2011. Blood. 117: 5463-5472. (in vivo depletion)Nguyen V, Cao L, Lin JT, Hung N, Ritz A, Yu K, Jianu R, Ulin SP, Raphael BJ, Laidlaw DH, Brossay L, and Salomon AR. 2009. Mol. Cell. Proeomics. 8: 2418-2431. (in vitro activation)Bibollet-Ruche F, McKinney BA, Duverger A, Wagner FH, Ansari AA, and Kutsch O. 2008. J. Virol. 82(20): 10271-10278. (in vitro activation – Chimpanzee)Roura-Mir C, Catalfamo M, Cheng TY, Marqusee E, Besra GS, Jaraquemada D, and Moody DB. 2005. J. Immunol. 174:3773-3780 (Immunohistochemistry – Acetone fixed frozen sections)Sato Y, Mukai K, Watanabe S, Goto M, and Shimosato Y. 1986. Am. J. Pathol. 125(3):431-435. (Immunohistochemistry – Paraffin embedded sections)

Limitations and Warranty

enQuire Bio’s Human Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD25 Antibody [PC61.5]

C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD4  and 0.125 ug APC Mouse Anti-CD25 (QAB34) (right panel) or 0.125 ug APC Rat IgG1 (left panel).

Mouse Anti-CD25 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD25 Antibody Clone: PC61.5
Clone PC61.5 Host and Isotype: Rat IgG1 lambda
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD25 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD25 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD25 could be seen inhematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD25 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Liang D, Zuo A, Shao H, Born WK, O’Brian R, Kaplan HJ, and Sun D. 2012. J. Immunol. 188: 5785-5791. (in vivo blocking)Yu P, Steel JC, Zhang M, Morris JC, Waitz R, Fasso M, Allison JP, and Waldmann TA. 2012. Proc. Natl. Acad. Sci. 109:6187-6192. (in vivo Treg depletion)Billiard F, Lobry C, Darrasse-Jeze G, Waite J, Liu et al. 2012. Blood. 119: 4656-4664. (in vivo Treg depletion)Tang S, Moore ML, Grayson JM and Dubey P. 2012. Cancer Res. 72: 1975-1985. (in vivo Treg depletion)Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).10F.9G2, J43, PC61 Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry – frozen tissue)Hashimoto N, Nabholz M, MacDonald HR, and Zubler RH. 1986. Eur. J. Immunol. 16(3): 317-320. (Blocking)Ceredig R, Lowenthal JW, Nabholz M, and MacDonald R. 1985. Nature. 314:98-100 (Immunohistochemistry)Lowenthal JW, Zulber RH, Nabholz M, and MacDonald HR. 1985. Nature. 315(6021): 669-672. (Immunoprecipitation, Blocking)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD25 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD3 Antibody [Hit3a]

Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD3 Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: Hit3a
Clone Hit3a Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD3 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3 could be seen in. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Lesourne R, Zvezdova E, Song K-D, El-Khoury D, Uehara S, Barr VA, Samelson LE and Love PE. 2012. J. Immunol. 189: 1154-1161. (in vitro activation)Knyazhitsky M, Moas E, Shaginov E, Luria A, and Braiman A. 2012. J. Biol. Chem. 287: 19725-19735. (in vitro activation)Ge Shuwang, Hertel B, Emden SH, Beneke J, Menne J, Haller H, and von Vietinghoff S. 2012. Nephrol. Dial. Transplant. 27: 2768-2772. (Immunofluorescence microscopy)Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM, and Varki A. 2010. J. Immunol. 184: 4185-4195. (Flow cytometry – Chimpanzee)Westermann J, Bode U, Sahle A, Speck U, Karin N, Bell EB, Kalies K, and Gebert A. 2005. J. Immunol. 174: 2517-2524. (Immunohistochemistry – frozen tissue)Mukouyama H, Janzen NK, Hernandez JM, Lam JS, Caliliw R, Wang AY, Figlin RA, Belldegrun AS, and Zeng G. 2004. Clin. Cancer Res. 10: 1421-1429. (in vitro blocking)

Limitations and Warranty

enQuire Bio’s Human Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD19 Antibody [HIB19]

Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD19 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD19 Antibody Clone: HIB19
Clone HIB19 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD19 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD19 / Leu-12 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD19 / Leu-12 could be seen in. We were unable to detect CD19 / Leu-12 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)So NSY, Ostrowski MA, and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (in vitro cell capture for microscopy)Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, Schlienger K, Toll A, Levine B, Rubin SC, and Coukos G. 2003. Clin. Cancer Res. 9: 264 – 272. (Immunohistochemistry)Hibe W, Dirnhofer S, Oberwasserlechner F, Eisterer W, Amman K, Schmid T, Hilbe G, Thaler J, and Woll E. 2003. J. Clin. Pathol. 56: 736-741. (Immunohistochemistry – frozen tissues)

Limitations and Warranty

enQuire Bio’s Human Anti-CD19 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD11c Antibody [N418]

C57Bl/6 splenocytes were stained with FITC Mouse Anti-MHC Class II  and 0.125 ug APC Mouse Anti-CD11c (QAB23) (right panel) or 0.125 ug APC Armenian Hamster IgG (left panel).

Mouse Anti-CD11c Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD11c Antibody Clone: N418
Clone N418 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11c Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11c antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD11c could be seen inhematopoietic cells in the bone marrow, lymphoid tissue in appendix, macrophages in lung and squamous epithelial cells in the tonsil. We were unable to detect CD11c in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Guerriero JL, Ditsworth D, Catanzaro JM, Sabino G, Furie MB, Kew RR, Crawford HC, and Zong W-X. 2011. J. Immunol. 186: 3517-3526. (Immunohistochemistry – paraffin embedded tissue)Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy – frozen tissue)Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, and Staunton DE. 2007. J. Leukoc. Biol. 81: 1395-1403. (in vitro blocking)Hagnerud, S, Manna PP, Cella M, Stenberg A, Frazier WA, Colonna M, and Oldenborg P-A. 2006. J. Immunol. 5772-5778. (Immunofluorescence microscopy – frozen tissue)Finkelman FD, Lees A, Birnbaum R, Gause WC, and Morris SC. 1996. J. Immunol. 157: 1406-1414. (in vivo activation)Huleatt JW and Lefrancois L. 1995. J. Immunol. 154: 5684-5693. (Immunoprecipitation)Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, and Steinman RM. 1990. J. Exp. Med. 171: 1753. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD11c Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD115 Antibody [AFS98]

Mouse Anti-CD115 / CSF1R / MCSF Receptor Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD115 / CSF1R / MCSF Receptor Antibody Clone: AFS98
Clone AFS98 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD115 / CSF1R / MCSF Receptor Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD115 / CSF1R / MCSF Receptor antibody reveals CD115 / CSF1R / MCSF Receptor expression is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD115 / CSF1R / MCSF Receptor expression as measured by anti-CD115 / CSF1R / MCSF Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++++++++++++++++++++
CSF1R Variability++++++++++++++++++++++++++++++

Selected References

Gautier EL, Chow A, Spanbroek R, Marcelin G, Greter M, Jakubzick C, Bogunovic M, Leboeuf M, van Rooijen N, Habenicht AJ, Merad M, and Randolph GJ. 2012. J. Immunol. 189: 2614-2624. (Flow cytometry)Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato, S, DeLuca HF, Suda T, Udagawa N, and Takahashi N. Proc. Natl. Acad. Sci. 109: 10006-10011. (in vitro blocking)Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T, and Kubota Y. 2011. Blood. 117: 5264-5272. (in vivo blocking)Fixley FJ, Xiong Y, Yu R Y-L, Sahai EA, Stanley ER, and Ye BH. 2005. J. Cell Sci. 118: 1873-1883. (Western blot)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD115 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD38 Antibody [HIT2]

Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD38 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD38 Antibody Clone: HIT2
Clone HIT2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5ul /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD38 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD38 antibody reveals CD38 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD38 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the prostate and seminal vesicle and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in glandular cells in the prostate and seminal vesicle and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD38 could be seen in. We were unable to detect CD38 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD38 expression as measured by anti-CD38 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity
CD38 Variability+++++++++++++++++++++

Selected References

Dianzani U, and Malavasi F. 1995. Crit. Rev. Immunol. 15(2):167-200. Mehta K, Shahid U, and Malavasi F. Oct. 1996. FASEB J. 10(12):1408-1417. Ferrero E, and Malavas F. Feb. 1999. J. Jeukoc. Biol. 65(2):151-161. Matrai Z. Feb. 2005. Hematology. 10(1):39-46.

Limitations and Warranty

enQuire Bio’s Human Anti-CD38 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD314 Antibody [CX5]

Mouse Anti-Anti-CD314 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD314 Antibody Clone: CX5
Clone CX5 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD314 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KLRK1 antibody staining is expected to be primarily localized to the plasma membrane.

Selected References

Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH and Lanier LL. 2000. Immunity. 12(6): 721-727. Carayannopoulos LN, Naidenko OV, Fremont DH and Yokoyama WM. 2002. J Immunol. 169(8): 4079-4083.Hamerman JA, Ogasawara K and Lanier LL. 2004. J Immunol. 172(4): 2001-2005. (Flow cytometry)Ogawawara K, Mamerman JA, Hsin H, Chikuma S, Bour-Jordan H, Chen T, Pertel T, Carnaud C, Bluestone JA and Lanier LL. 2003. Immunity. 18: 41-51. (in vivo blocking)Andre MC, Siqurdardottir D, Kuttruff S, Pommerl B, Handgretinger R, Rammensee HG and Steinle A. 2012. Int J Cancer. 131(7): 1601-1610. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD314 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45RA Antibody [HI100]

Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD45RA Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD45RA Antibody Clone: HI100
Clone HI100 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45RA Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RA could be seen in. We were unable to detect CD45RA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RA expression as measured by anti-CD45RA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++
PTPRC Variability++++++++++++++++++++

Selected References

Kroenke MA, Eto, D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow Cytometry) Lopez-Verges S, Milush JM, Schwartz BS, Pando MJ, Jarjoura J, York VA, Houchins JP, Miller S, Kang S-M, Norris PJ, Nixon DF, and Lanier LL. 2011. Proc. Natl. Acad. Sci. 108: 14725-14732. (Flow Cytometry)Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. (Immunohistochemistry – paraffin embedded tissue)Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (Western Blot)Weninger W, Carlsen HS, Goodarzi M, Moazed F, Crowley MA, Baekkevold ES, Cavanagh LL, and von Andrian U. 2003. J. Immunol. 170: 4638-4648. (Immunohistochemistry – frozen tissue.Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)

Limitations and Warranty

enQuire Bio’s Human Anti-CD45RA Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45.1 Antibody [A20]

C57Bl/6  (solid line) or 0.5 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD45.1 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD45.1 Antibody Clone: A20
Clone A20 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45.1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45.1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45.1 could be seen in. We were unable to detect CD45.1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45.1 expression as measured by anti-CD45.1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++
PTPRC Variability++++++++++++++++++++

Selected References

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD45.1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Foxp3 Antibody [MF23]

Mouse Anti-FOXP3 / Scurfin Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-FOXP3 / Scurfin Antibody Clone: MF23
Clone MF23 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

FOXP3 / Scurfin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-FOXP3 / Scurfin antibody reveals FOXP3 / Scurfin expression is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry) Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry – Rhesus macaque)Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-Foxp3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD5 Antibody [UCHT2]

Human PBMCs were stained with 5 uL  (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD5 Antibody Product Attributes

Species: Human
Cross Reactivity: capuchin monkey, chimpanzee, common marmoset
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: UCHT2
Clone UCHT2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6): 1630-1634. Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223): 535-537. Wood GS and Freudenthal PS. 1992. Am J Pathol. 141(4): 789-795. (Flow Cytometry)

Limitations and Warranty

enQuire Bio’s Human Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD4 Antibody [SK3]

Human PBMCs were stained with 5 uL  (solid line) or 0.06 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD4 Antibody Product Attributes

Species: Human
Cross Reactivity: cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: SK3
Clone SK3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM and Good RA. 1981. Proc Natl Acad Sci U S A. 78(1): 544-548.Sattentau QJ, Dalgleish AG, Weiss RA and Beverley PC. 1986. Science. 234(4780): 1120-1123. Bernard A, Boumsell L and Hill C. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984: 9-108. (Flow Cytometry) Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E and Monti P. 2012. J Immunol. 189(12): 5649-5648. (Flow Cytometry) Yoshino N, Ami Y, Terao K, Tashiro F and Honda M. 2000. Exp. Anim. 49(2): 97-110. (Flow Cytometry – Cynomolgus) Lafont BAP, Gloeckler L, D’Hautcourt JL, Gut JP and Aubertin AM. 2000. Cytometry 41: 193-202. (Flow Cytometry – Rhesus)

Limitations and Warranty

enQuire Bio’s Human Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-TIGIT Antibody [1G9]

Mouse Anti-TIGIT / VSTM3 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-TIGIT / VSTM3 Antibody Clone: 1G9
Clone 1G9 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

TIGIT / VSTM3 Previously Observed Antibody Staining Patterns

Selected References

Joller N, Peters A, Anderson AC, and Kuchroo VK. 2012. Immunol. Rev. 248(1):122-139. (Flow cytometry)Joller N, Hafler JP, Brynedal B, Kassam N, Spoerl S, Levin SD, Sharpe AH, and Kuchroo VK. 2011. J. Immunol. 186: 1338-1342. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-TIGIT Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-p40 Antibody Clone

Formalin-fixed, paraffin-embedded Prostate Carcinoma stained with p4 Rabbit Polyclonal Antibody.

Human, Mouse, Rat, and Cow Anti-p40 Antibody Product Attributes

Species: Human, Mouse, Rat, and Cow
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Polyclonal
Anti-p40 Antibody Clone: polyclonal
Clone Host and Isotype: Rabbit IgG
Anti-Human, Mouse, Rat, and Cow p40 Positive Control Sample: HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma
Cellular Localization of Antibody < Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p40 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TP63 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in p40 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. Low, but measureable presence of p40 could be seen inglandular cells in the breast, cervix, uterine. We were unable to detect p40 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p40 expression as measured by anti-p40 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity++++++++++++++++
TP63 Variability++++++++++++++++++++++++++++++

Limitations and Warranty

enQuire Bio’s p40 Anti-Human Polyclonal Anti-Human Polyclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD10 Antibody [SN5c]

Human peripheral blood granulocytes were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD10 / Neprilysin / MME Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD10 / Neprilysin / MME Antibody Clone: SN5c
Clone SN5c Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD10 / Neprilysin / MME Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD10 / Neprilysin / MME antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the tubules in kidney and glandular cells in the duodenum, prostate and small intestine. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the tubules in kidney and glandular cells in the duodenum, prostate and small intestine. Low, but measureable presence of CD10 / Neprilysin / MME could be seen inmacrophages in lung. We were unable to detect CD10 / Neprilysin / MME in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP and Seon BK. 1987. Cancer Res. 47(8): 2160-2166. Shipp MA, Stefano GB, Switzer SN, Griffin JD and Reinherz EL. 1991. Blood. 78(7): 1834-1841. Pinho S, Lacombe J, Hanoun M, Mizoguchi T, Bruns I, Kunisaki Y and Frenette PS. 2013. J Exp Med. 201(7): 1351-1367. (Flow cytometry) Mukhopadhyay C, Zhao X, Maroni D, Band V and Naramura M. 2013. PLoS One. 8(10): e75907. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Human Anti-CD10 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD14 Antibody [61D3]

Human peripheral blood monocytes were stained with 5 uL APC conjugated anti-CD14 antibody (solid line) or 1 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD14 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 µl per test where one test represents staining of a cell sample in a final volume of approximately 100 µL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD14 Antibody Clone: 61D3
Clone 61D3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2
Antibody Concentration: 5 &microL/test
Storage Conditions: 2-8°C protected from light. Stable for 12 Months. Do Not Freeze Conjugated Formats.

CD14 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD14 antibody reveals CD14 expression is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD14 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon. More moderate antibody staining intensity was present in endothelial cells in the colon. Low, but measureable presence of CD14 could be seen in cells in the seminiferous ducts in testis, fibroblasts in skin, glandular cells in the fallopian tube and salivary gland, Langerhans in skin and Leydig cells in the testis. We were unable to detect CD14 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD14 expression as measured by anti-CD14 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity
CD14 Variability++++++++++++++++++++++++

Selected References

Hogg N, Horton MA. 1987. In: McMichael AJ, Beverley PCL, Cobbold S, et al., eds. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 576-602. Haziot A, Chen S, Ferrero E, Low MG, Silber R, Goyert SM. 1988. J Immunol. 141:547-552.

Limitations and Warranty

enQuire Bio’s Human Anti-CD14 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD161 Antibody [PK136]

C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3e  and 0.125 ug APC Mouse Anti-NK1.1 (CD161) (QAB78) (right panel) or 0.125 ug APC Mouse IgG2a isotype control (left panel).

Mouse Anti-Anti-CD161 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD161 Antibody Clone: PK136
Clone PK136 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD161 Previously Observed Antibody Staining Patterns

Selected References

Krebs DL, Chehal MK, Sio A, Huntington ND, Da ML, Ziltener P, Inglese M, Kountouri N, Priatel JJ, Jones J, Tarlinton DM, Anderson GP, Hibbs ML, and Harder KW. 2012. J. Immunol. 188:5094-5105. (in vivo depletion)Lubinski JM, Lazear HM, Awasthi S, Wang F, and Friedman HM. 2011. J. Virol. 85(7): 3239-3249. (in vivo depletion)Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vivo depletion)Awasthi A, Samarakoon A, Chu H, Kamalakannan R, Quilliam LA, Chrzanowska-Wodnicka M, White GC, and Malarkannan S. 2010. J. Exp. Med. 207: 1923-1938. (in vitro activation)Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation) Ljutic B, Carlyle JR, Filipp D, Nakagawa R, Julius M, and Zuniga-Pflucker JC. 2005. J. Immunol. 174: 4789-4796. (Immunoprecipitation) Kanwar JR, Shen W-P, Kanwar RK, Berg RW, and Krissansen GW. 2001. J. Natl. Cancer Inst. 93: 1541-1552. (Immunohistochemistry – frozen tissue, Immunofluorescence microscopy – frozen tissue, in vivo depletion)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD161 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD154 Antibody [MR1]

Mouse Anti-Anti-CD154 / TNFSF5 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD154 / TNFSF5 Antibody Clone: MR1
Clone MR1 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD154 / TNFSF5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD40L / CD154 / TNFSF5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD40L / CD154 / TNFSF5 could be seen inhematopoietic cells in the bone marrow. We were unable to detect CD40L / CD154 / TNFSF5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. 1992. Proc Natl Acad Sci U S A. 89(14):6550-6554.Foy TM, Laman JD, Ledbetter JA, Aruffo A, Claassen E, Noelle RJ. 1994. J Exp Med. 180(1):157-163.Laman JD, Claassen E, Noelle RJ. 1996. Crit Rev Immunol. 16(1):59-108.Grewal IS, Flavell RA. 1998. Annu Rev Immunol. 16:111-35.

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD154 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD154 Antibody [MR1]