Melanoma Cocktail Antibody [HMB45+A103+T311] Antibody (cGMP).

Immunohistochemical staining of Melanoma Cocktail Antibody  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:25-1:50
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Melanoma Cocktail Antibody Antibody Clone: HMB45+A103+T311
Host and Isotype: Mouse HMB45 and A103, IgG1; T311, IgG2a
Recommended Positive Control Sample: Metastatic melanoma in lymph node
Cellular Localization of Antibody HMB45+A103+T311 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Melanoma Cocktail Antibody Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Anti-CD11c Antibody [N418]

C57Bl/6 splenocytes were stained with FITC Mouse Anti-MHC Class II  and 0.125 ug APC Mouse Anti-CD11c (QAB23) (right panel) or 0.125 ug APC Armenian Hamster IgG (left panel).

Mouse Anti-CD11c Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD11c Antibody Clone: N418
Clone N418 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11c Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11c antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD11c could be seen inhematopoietic cells in the bone marrow, lymphoid tissue in appendix, macrophages in lung and squamous epithelial cells in the tonsil. We were unable to detect CD11c in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Guerriero JL, Ditsworth D, Catanzaro JM, Sabino G, Furie MB, Kew RR, Crawford HC, and Zong W-X. 2011. J. Immunol. 186: 3517-3526. (Immunohistochemistry – paraffin embedded tissue)Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy – frozen tissue)Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, and Staunton DE. 2007. J. Leukoc. Biol. 81: 1395-1403. (in vitro blocking)Hagnerud, S, Manna PP, Cella M, Stenberg A, Frazier WA, Colonna M, and Oldenborg P-A. 2006. J. Immunol. 5772-5778. (Immunofluorescence microscopy – frozen tissue)Finkelman FD, Lees A, Birnbaum R, Gause WC, and Morris SC. 1996. J. Immunol. 157: 1406-1414. (in vivo activation)Huleatt JW and Lefrancois L. 1995. J. Immunol. 154: 5684-5693. (Immunoprecipitation)Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, and Steinman RM. 1990. J. Exp. Med. 171: 1753. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD11c Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD115 Antibody [AFS98]

Mouse Anti-CD115 / CSF1R / MCSF Receptor Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD115 / CSF1R / MCSF Receptor Antibody Clone: AFS98
Clone AFS98 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD115 / CSF1R / MCSF Receptor Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD115 / CSF1R / MCSF Receptor antibody reveals CD115 / CSF1R / MCSF Receptor expression is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD115 / CSF1R / MCSF Receptor expression as measured by anti-CD115 / CSF1R / MCSF Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++++++++++++++++++++
CSF1R Variability++++++++++++++++++++++++++++++

Selected References

Gautier EL, Chow A, Spanbroek R, Marcelin G, Greter M, Jakubzick C, Bogunovic M, Leboeuf M, van Rooijen N, Habenicht AJ, Merad M, and Randolph GJ. 2012. J. Immunol. 189: 2614-2624. (Flow cytometry)Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato, S, DeLuca HF, Suda T, Udagawa N, and Takahashi N. Proc. Natl. Acad. Sci. 109: 10006-10011. (in vitro blocking)Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T, and Kubota Y. 2011. Blood. 117: 5264-5272. (in vivo blocking)Fixley FJ, Xiong Y, Yu R Y-L, Sahai EA, Stanley ER, and Ye BH. 2005. J. Cell Sci. 118: 1873-1883. (Western blot)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD115 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD38 Antibody [HIT2]

Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD38 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD38 Antibody Clone: HIT2
Clone HIT2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5ul /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD38 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD38 antibody reveals CD38 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD38 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the prostate and seminal vesicle and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in glandular cells in the prostate and seminal vesicle and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD38 could be seen in. We were unable to detect CD38 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD38 expression as measured by anti-CD38 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity
CD38 Variability+++++++++++++++++++++

Selected References

Dianzani U, and Malavasi F. 1995. Crit. Rev. Immunol. 15(2):167-200. Mehta K, Shahid U, and Malavasi F. Oct. 1996. FASEB J. 10(12):1408-1417. Ferrero E, and Malavas F. Feb. 1999. J. Jeukoc. Biol. 65(2):151-161. Matrai Z. Feb. 2005. Hematology. 10(1):39-46.

Limitations and Warranty

enQuire Bio’s Human Anti-CD38 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD314 Antibody [CX5]

Mouse Anti-Anti-CD314 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD314 Antibody Clone: CX5
Clone CX5 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD314 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KLRK1 antibody staining is expected to be primarily localized to the plasma membrane.

Selected References

Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH and Lanier LL. 2000. Immunity. 12(6): 721-727. Carayannopoulos LN, Naidenko OV, Fremont DH and Yokoyama WM. 2002. J Immunol. 169(8): 4079-4083.Hamerman JA, Ogasawara K and Lanier LL. 2004. J Immunol. 172(4): 2001-2005. (Flow cytometry)Ogawawara K, Mamerman JA, Hsin H, Chikuma S, Bour-Jordan H, Chen T, Pertel T, Carnaud C, Bluestone JA and Lanier LL. 2003. Immunity. 18: 41-51. (in vivo blocking)Andre MC, Siqurdardottir D, Kuttruff S, Pommerl B, Handgretinger R, Rammensee HG and Steinle A. 2012. Int J Cancer. 131(7): 1601-1610. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD314 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45RA Antibody [HI100]

Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD45RA Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD45RA Antibody Clone: HI100
Clone HI100 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45RA Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RA could be seen in. We were unable to detect CD45RA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RA expression as measured by anti-CD45RA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++
PTPRC Variability++++++++++++++++++++

Selected References

Kroenke MA, Eto, D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow Cytometry) Lopez-Verges S, Milush JM, Schwartz BS, Pando MJ, Jarjoura J, York VA, Houchins JP, Miller S, Kang S-M, Norris PJ, Nixon DF, and Lanier LL. 2011. Proc. Natl. Acad. Sci. 108: 14725-14732. (Flow Cytometry)Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. (Immunohistochemistry – paraffin embedded tissue)Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (Western Blot)Weninger W, Carlsen HS, Goodarzi M, Moazed F, Crowley MA, Baekkevold ES, Cavanagh LL, and von Andrian U. 2003. J. Immunol. 170: 4638-4648. (Immunohistochemistry – frozen tissue.Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)

Limitations and Warranty

enQuire Bio’s Human Anti-CD45RA Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45.1 Antibody [A20]

C57Bl/6  (solid line) or 0.5 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD45.1 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD45.1 Antibody Clone: A20
Clone A20 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45.1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45.1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45.1 could be seen in. We were unable to detect CD45.1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45.1 expression as measured by anti-CD45.1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Typebreast cancercarcinoidcervical cancercolorectal cancerendometrial cancergliomahead and neck cancerliver cancerlung cancerlymphomamelanomaovarian cancerpancreatic cancerprostate cancerrenal cancerskin cancerstomach cancertesticular cancerthyroid cancerurothelial cancer
Signal Intensity+++
PTPRC Variability++++++++++++++++++++

Selected References

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio’s Mouse Anti-CD45.1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Foxp3 Antibody [MF23]

Mouse Anti-FOXP3 / Scurfin Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-FOXP3 / Scurfin Antibody Clone: MF23
Clone MF23 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

FOXP3 / Scurfin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-FOXP3 / Scurfin antibody reveals FOXP3 / Scurfin expression is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry) Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry – Rhesus macaque)Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-Foxp3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD5 Antibody [UCHT2]

Human PBMCs were stained with 5 uL  (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD5 Antibody Product Attributes

Species: Human
Cross Reactivity: capuchin monkey, chimpanzee, common marmoset
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: UCHT2
Clone UCHT2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6): 1630-1634. Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223): 535-537. Wood GS and Freudenthal PS. 1992. Am J Pathol. 141(4): 789-795. (Flow Cytometry)

Limitations and Warranty

enQuire Bio’s Human Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD4 Antibody [SK3]

Human PBMCs were stained with 5 uL  (solid line) or 0.06 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD4 Antibody Product Attributes

Species: Human
Cross Reactivity: cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: SK3
Clone SK3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM and Good RA. 1981. Proc Natl Acad Sci U S A. 78(1): 544-548.Sattentau QJ, Dalgleish AG, Weiss RA and Beverley PC. 1986. Science. 234(4780): 1120-1123. Bernard A, Boumsell L and Hill C. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984: 9-108. (Flow Cytometry) Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E and Monti P. 2012. J Immunol. 189(12): 5649-5648. (Flow Cytometry) Yoshino N, Ami Y, Terao K, Tashiro F and Honda M. 2000. Exp. Anim. 49(2): 97-110. (Flow Cytometry – Cynomolgus) Lafont BAP, Gloeckler L, D’Hautcourt JL, Gut JP and Aubertin AM. 2000. Cytometry 41: 193-202. (Flow Cytometry – Rhesus)

Limitations and Warranty

enQuire Bio’s Human Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-TIGIT Antibody [1G9]

Mouse Anti-TIGIT / VSTM3 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-TIGIT / VSTM3 Antibody Clone: 1G9
Clone 1G9 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

TIGIT / VSTM3 Previously Observed Antibody Staining Patterns

Selected References

Joller N, Peters A, Anderson AC, and Kuchroo VK. 2012. Immunol. Rev. 248(1):122-139. (Flow cytometry)Joller N, Hafler JP, Brynedal B, Kassam N, Spoerl S, Levin SD, Sharpe AH, and Kuchroo VK. 2011. J. Immunol. 186: 1338-1342. (Flow cytometry)

Limitations and Warranty

enQuire Bio’s Mouse Anti-TIGIT Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Inhibin [R1] Antibody (cGMP).

Immunohistochemical staining of Inhibin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:25-1:50
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Inhibin Antibody Clone: R1
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Ovary
Cellular Localization of Antibody R1 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Synthetic peptide corresponding to the 1-32 amino terminal peptide of the alpha subunit of human inhibin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Inhibin Information for Pathologists

Summary:

Inhibin is a heterodimeric protein (with alpha and beta subunits) that inhibits or activates pituitary FSH secretion. Serum levels elevated (2x) in women with Down fetus. Positive staining – normal Sertoli cells (diffuse and strong), granulosa cells, prostate, brain, adrenal. Positive staining – disease

Common Uses By Pathologists:

Normal sertoli cells (diffuse and strong), granulosa cells, prostate, brain, and adrenal as well as Sex-cord stromal tumors, including Sertoli cell tumors, adrenocortical tumors, placental and gestational trophoblastic lesions, granular cell tumors of gallbladder and extrahepatic bile ducts (Am J Surg Pathol 2001;25:1200), and some carcinomas stain positive for Inhibin A. Primary ovarian carcinomas are usually negative for the protein.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Myeloperoxidase [Polyclonal] Antibody (cGMP).

Immunohistochemical staining of Myeloperoxidase  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:25-1:100
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-Myeloperoxidase Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Myeloperoxidase isolated from human granulocytes.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Myeloperoxidase Information for Pathologists

Summary:

Staining is either immunohistochemistry (IHC) or enzyme cytochemistry – enzyme cytochemistry is most sensitive and specific stain for myeloid leukemias and granulocytic sarcoma. Stains neutrophils strongly (diffuse granular pattern), other granulocytes variably. Microscopic (histologic) images Missing Image. Myeloid sarcoma of breast.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Vimentin [V9] Antibody (cGMP).

Immunohistochemical staining of Vimentin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:25-1:50
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Vimentin Antibody Clone: V9
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Sarcoma, tonsil
Cellular Localization of Antibody V9 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with purified vimentin from porcine eye lens.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Vimentin Information for Pathologists

Summary:

Intermediate filament for mesenchymal tissue. Uses by pathologists Vimentin staining confirms mesenchymal origin of some tumors; may be the only positive stain and thus confirms that the tissue is capable of staining (Am J Surg Pathol 2011;35:1722). Staining may confirm mesenchymal origin, but numerous exceptions so relatively nonspecific. Help distinguish renal cell clear cell carcinoma from mimics as part of panel.

Common Uses By Pathologists:

Vimentin staining confirms mesenchymal origin of some tumors; may be the only positive stain and thus confirms that the tissue is capable of staining (Am J Surg Pathol 2011;35:1722). Staining may confirm mesenchymal origin, but numerous exceptions so relatively nonspecific. Help distinguish renal cell clear cell carcinoma from mimics as part of panel. Absence of vimentin staining may confirm that fat-like or other spaces actually lack a cellular lining (Am J Surg Pathol 2011;35:1823). Distinguish endocervical and endometrial adenocarcinoma, as part of panel (Am J Surg Pathol 2010;34:915).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD23 [SP23] Antibody (cGMP).

Immunohistochemical staining of CD23  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:50-1:100
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at RoomTemperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD23 Antibody Clone: SP23
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody SP23 Staining: Cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant protein encoding human CD23 48-248 aa.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD23 Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD25 [EP218] Antibody (cGMP).

Immunohistochemical staining of CD25  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:25-1:50
PretreatmentEDTA Buffer pH 8.0
Incubation Parameters30 min at RoomTemperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD25 Antibody Clone: EP218
Host and Isotype: Rabbit IgG
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody EP218 Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues of human CD25 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD25 Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD5 [4C7] Antibody (cGMP).

Immunohistochemical staining of CD5  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:10-1:20
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD5 Antibody Clone: 4C7
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody 4C7 Staining: Cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant prokaryotic fusion protein corresponding to the external domain of the CD5 molecule.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD5 Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD8 [SP16] Antibody (cGMP).

Immunohistochemical staining of CD8  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:50-1:100
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD8 Antibody Clone: SP16
Host and Isotype: Rabbit IgG
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody SP16 Staining: Cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of the CD8 alpha chain.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD8 Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Calcitonin [SP17] Antibody (cGMP).

Immunohistochemical staining of Calcitonin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:50-1:100
PretreatmentCitrate Buffer pH 6.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Calcitonin Antibody Clone: SP17
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Thyroid or medullary carcinoma
Cellular Localization of Antibody SP17 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Synthetic human calcitonin 1-32 amino acid peptide.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Calcitonin Information for Pathologists

Summary:

Calcitonin (thyrocalcitonin) is a 32 amino acid linear polypeptide hormone (molecular weight 3,421Kd) that participates in calcium and phosphorus metabolism. It is formed by the proteolytic cleavage of a larger pre-propeptide, which is a product of the CALC1 gene (CALC). In humans, it is primarily produced by the parafollicular cells (C cells) of the thyroid. Other tissues such as the lungs and intestinal tract can also synthesize calcitonin. The calcitonin receptor protein has been shown to be a member of the seven transmembrane G protein coupled receptor family, which is coupled by G5 to adenylate cyclase and thereby to the generation of CAMP in target cells.

Common Uses By Pathologists:

As a marker for medullary thyroid carcinoma (MTC), especially when facing histologic subtypes such as the follicular, papillary or encapsulated variants that can pose diagnostic difficulties with follicular cellĀ–derived carcinomas and paraganglioma (Arch Pathol Lab Med 2008;132:359). Although highly specific, calcitonin staining may be patchy in medullary carcinomas and 5% of these tumors may be negative for this marker. In tumors with no staining and still suspected to be MTC, calcitonin and calcitonin geneĀ–related peptide mRNA can be demonstrated by in situ hybridization (Arch Pathol Lab Med 2010;134:207). To evaluate for C cell hyperplasia associated with familial MTC. High grade neuroendocrine tumors can also stain with calcitonin, and metastatic lesions can be misinterpreted as having arisen in the thyroid (Adv Anat Pathol 2004;11:202).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Caldesmon [hHCD] Antibody (cGMP).

Immunohistochemical staining of Caldesmon  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
SpecificationRecommendation
Recommended Dilution (Conc)1:50-1:100
PretreatmentEDTA Buffer pH 8.0
Incubation Parameters30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Caldesmon Antibody Clone: hHCD
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Endometrial cancer
Cellular Localization of Antibody hHCD Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with human uterus smooth muscle extract.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Caldesmon Information for Pathologists

Summary:

Caldesmon binds calcium, calmodulin, tropomyosin and actin and regulates smooth muscle contraction (Int J Biochem Cell Biol 1997;29:1047). Regulates smooth muscle contraction and relaxation. May play a role in preventing unwanted contractions (Am J Physiol Heart Circ Physiol 2009;297:H1930). Interpretation: cytoplasmic staining. h-caldesmon is high molecular weight isoform, found in smooth muscle cells; l-caldesmon isoform is distributed in non-muscle cells (J Biol Chem 1991;266:355).

Common Uses By Pathologists:

Very useful to distinguish smooth muscle cells / lesions (positive) from myofibroblastic cells / lesions (negative or only a few cells positive, Am J Clin Pathol 2000;114:746, Am J Dermatopathol 2006;28:105). Distinguish uterine leiomyoma or leiomyosarcoma (positive) from endometrial stromal tumors (negative, Am J Surg Pathol 2001;25:455), but endometrial stromal tumors may have focal smooth muscle differentiation (Am J Surg Pathol 2002;26:1142). Sensitive and specific for epithelioid peritoneal mesothelioma (positive) versus ovarian serous carcinoma (negative, Am J Surg Pathol 2007;31:1139) and epithelioid pleural mesothelioma (positive) versus pulmonary adenocarcinoma (negative, Am J Surg Pathol 2006;30:463). Microscopic (histologic) images Images hosted on other servers:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Caldesmon [hHCD] Antibody (cGMP).