Human Anti-CD11b Antibody Product Attributes
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD11b Antibody Clone: ICRF44
Clone ICRF44 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD11b Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD11b antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD11b could be seen in cells in the molecular layer in cerebellum, glial cells in the caudate nucleus, cerebral cortex and hippocampus and macrophages in lung. We were unable to detect CD11b in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, and Cross AS. 2011. J. Leukoc. Biol. 90:313-321. (Immunoprecipitation)Chang WLW and Barry PA. 2010. Proc. Natl. Acad. Sci. 107:22647-2652. (Flow cytometry - Rhesus macaque)Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, and Kettritz R. 2010. J. Biol. Chem. 286:7070-7081. (in vitro blocking)Moreau A, Hill M, Thebault P, Deschamps JY, Chiffoleau E, Chauveau C, Moullier P, Anegon I, Alliot-Licht B, and Cuturi MC. 2009. FASEB J. 23:3070-3077. (Flow cytometry - cynomolgus macaque)Sengoku K, Takuma N, Miyamoto T, Horikawa M, and Ishikawa M. 2004. Hum. Reprod. 19: 639-644. (Immunofluorescence microscopy)David A, Kacher Y, Specks U, and Aviram I. 2003. J. Leukoc. Biol. 74:551-557. (Western blot)Rezzonico R, Imbert V, Chicheportiche R, and Dayer J-M. 2001. Blood. 97: 2932-2940. (in vitro activation)
Limitations and Warranty
enQuire Bio's Human Anti-CD11b Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.