Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD3 Antibody [UCHT1]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;Biotin ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB6-F-25Tests
CD3 General Information
Alternate Names
Leu-4, T3
Curated Database and Bioinformatic Data
Gene SymbolCD3D
Entrez Gene ID915
Ensemble Gene IDENSG00000167286
RefSeq Protein Accession(s)XP_016874032, NP_000723, NP_001035741
RefSeq mRNA Accession(s)NM_000732, NM_001040651
RefSeq Genomic Accession(s)NC_000011, NG_009891, NC_018922
UniProt ID(s)B0YIY4, P04234
UniGene ID(s)B0YIY4, P04234
HGNC ID(s)1673
Cosmic ID(s)CD3D
KEGG Gene ID(s)hsa:915
PharmGKB ID(s)PA26215
General Description of CD3.
The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, Hit3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry; however the antibody is reported to be unsuitable for induction of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).

Human Anti-CD3 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: UCHT1
Clone UCHT1 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD3 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3 could be seen in. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD3 expression as measured by anti-CD3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - ++ - - - - - - - - - -
CD3D Variability + + + + + ++ + ++ + ++ + + ++ + + + + ++ ++ +

Selected References

Harris SJ, Parry RV, Foster JG, Blunt MD, Wang A, Marelli-Berg F, Westwick J, and Ward SG. Apr. 2011. J. Immunol. 186: 4936-4945. (in vitro activation)Beriou G, Bradshaw EM, Lozano E, Costantino CM, Hastings WD, Orban T, Elyaman W, Khoury SJ, Kuchroo VK, Baecher-Allan C, and Hafler DA. 2010. J. Immunol. 185: 46-54. (in vitro activation)Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM, and Varki A. 2010. J. Immunol. 184: 4185-4195. (Flow cytometry - Chimpanzee)Edelbauer M, Datta D, Vos IHC, Basu A, Stack MP, Reinders MEJ, Sho M, Calzadilla K, Ganze P, and Briscoe DM. 2010. Blood. 116:1980-1989. (Immunohistochemistry - acetone fixed, frozen sections; Immunofluoresence microscopy)Varghese JC and Kane KP. 2008. J. Immunol. 181: 6002-6009. (in vitro activation)Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1):79-87. (Immunohistochemistry - frozen tissue)Sakkas LI, Scanzello C, Johanson N, Burkholder J, Mitra A, Salgame P, Katsetos CD, and Platsoucas CD. 1998. Clin. Diagn. Lab. Immun. 5:430. (Immunohistochemistry - acetone fixed, frozen sections)Salmeron A, Sanchez-Madrid F, Ursa MA, Fresno M, and Alarcon B. 1991. J. Immunol. 147:3047-3052. (Immunoprecipitation)Van Dongen JJ, Krissansen GW, Wolvers-Tettero IL, Comans-Bitter WM, Adriaansen HJ, Hooijkaas H, van Wering ER, and Terhorst C. 1988. Blood. 71: 603-612. (Western Blot)

Limitations and Warranty

enQuire Bio's Human Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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