C57Bl/6 splenocytes were stained with 0.5 ug APC Mouse Anti-CD45.2 (QAB43) (solid line) or 0.5 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD45.2 Antibody [104]

$ 49.00$ 239.00
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SKU: QAB43
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB43-F-25ug
CD45.2 General Information
Alternate Names
CD45, CD45 allele 2
Curated Database and Bioinformatic Data
Gene SymbolPtprc
Entrez Gene ID19264
Ensemble Gene IDENSMUSG00000026395
RefSeq Protein Accession(s)NP_035340, XP_006529324, XP_006529321, XP_006529323, NP_001104786, XP_006529320, XP_006529322, XP_006529325, NP_001255215
RefSeq mRNA Accession(s)XM_006529259, XM_006529262, NM_011210, XM_006529260, NM_001111316, XM_006529257, XM_006529258, XM_006529261, NM_001268286
RefSeq Genomic Accession(s)NC_000067
UniProt ID(s)S4R1M0, S4R1S4, S4R2V1, Q8C6Q7
UniGene ID(s)S4R1M0, S4R1S4, S4R2V1, Q8C6Q7
Cosmic ID(s)Ptprc
KEGG Gene ID(s)mmu:19264
General Description of CD45.2.
The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

Mouse Anti-CD45.2 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD45.2 Antibody Clone: 104
Clone 104 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45.2 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45.2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45.2 could be seen in. We were unable to detect CD45.2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45.2 expression as measured by anti-CD45.2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Selected References

Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry)Hale JS, Nelson LT, Simmons KB, and Fink PJ. 2011. J. Immunol. 186: 799 - 806. (Flow Cytometry)Orr MT, Beilke JN, Proekt I, and Lanier LL. 2010. Proc. Natl. Acad. Sci. 107: 15844 - 15849. (Flow Cytometry)Banerjee K, Biswas PS, Kumaraguru U, Schoenberger SP, and Rouse BT. 2004. 173: 7575-7583. (Immunofluorescence microscopy - frozen tissue)Favre CJ, Mancuso M, Maas K, McLean JW, Baluk P, and Mcdonald DM. 2003. Am. J. Physiol. Heart Circ. Physiol. 285:H1917-H1938. (Immunocytochemistry)Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation).

Limitations and Warranty

enQuire Bio's Mouse Anti-CD45.2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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