C57Bl/6 splenocytes were stained with 0.25 ug APC Mouse Anti-CD5 (QAB13) (solid line) or 0.25 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD5 Antibody [53-7.3]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PE
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB13-F-25ug
CD5 General Information
Alternate Names
CD5, LEU1, Ly-1, Ly-12, Ly-A, Lyt-1
Curated Database and Bioinformatic Data
Gene SymbolCd5
Entrez Gene ID12507
Ensemble Gene IDENSMUSG00000024669
RefSeq Protein Accession(s)NP_031676, XP_006526684
RefSeq mRNA Accession(s)XM_006526621, NM_007650
RefSeq Genomic Accession(s)NC_000085
UniProt ID(s)Q3UP78, P13379
UniGene ID(s)Q3UP78, P13379
Cosmic ID(s)Cd5
KEGG Gene ID(s)mmu:12507
General Description of CD5.
The 53-7.3 monoclonal antibody is specific for mouse CD5, a 67 kDa protein that is expressed on most thymocytes, mature T cells and the B-1 subset of B cells. CD5 is a member of the scavenger receptor cysteine-rich protein superfamily and is a ligand for the c-type lectin CD72. CD5 is also known as Leu-1 and functions to modulate antigen receptor signaling on T and B cells.

Mouse Anti-CD5 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: 53-7.3
Clone 53-7.3 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Azzam HS, Grinberg A, Lui K, Shen H, Shores EW, Love PE. 1998. J Exp Med. 188(12):2301-2311.Bikah G, Carey J, Ciallella JR, Tarakhovsky A, Bondada S. 1996. Science. 274(5294):1906-1909.Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. 1998. Int Immunol. 10(8):1185-1196.Hayakawa K, Hardy RR, Parks DR, Herzenberg LA. 1983. J Exp Med. 157(1):202-218.Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. 1980. J Exp Med. 152(2):280-295.Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223):535-537.

Limitations and Warranty

enQuire Bio's Mouse Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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