Human Anti-CD8 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: OKT8
Clone OKT8 Host and Isotype: Mouse IgG2a
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD8 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Jahnke M, Trowsdale J, and Kelly AP. 2012. J. Biol. Chem. 287: 28779-28789. (Flow Cytometry, Immunoprecipitation) Clement M, Ladell K, Ekeruche-Makinde J, Miles JJ, Edwards ESJ, Dolton G, Williams T, Schauenburg AJA, Cole DK, Lauder SN, Gallimore AM, Godkin AJ, Burrows SR, Price DA, Sewell AK, and Wooldridge L. 2011. J. Immunol. 187: 654-663. (in vitro activation) Bagnara D, Kaufman MS, Calissano C, Marsilio S, Patten PEM, Simone R, Chum P, Yan X-Y, Allen SL, Kolitz JE, Baskar S, Radar C, Mellstedt H, Rabbani H, Lee A, Gregersen PK, Rai KR, and Chiorazzi N. 2011. Blood. 117: 5463-5472. (in vivo activation) Teles RMB, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, and Modlin RL. 2010. 78: 4634-4643. (Immunohistochemistry - OCT embedded frozen tissue) Lai AY, Fatemi M, Dhasarathy A, Malone C, Sobol SE, Geigerman C, Jaye DL, Mav D, Shah R, Li L, and Wade PA. 2010. J. Exp. Med. 207: 1939-1950. (in vitro T cell depletion) Varghese JC and Kane KP. 2008. J. Immunol. 181: 6002-6009. (in vitro blocking) Thakral D, Dobbins J, Devine L, and Kavathas PB. 2008. J. Immunol. 180:7431-7442. (Immunoprecipitation)
Limitations and Warranty
enQuire Bio's Human Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.