C57Bl/6 splenocytes were stimulated with anti-IgM and anti-CD40 for 4 days. Cells were then stained with 0.06 ug APC Mouse Anti-CD80 (QAB50) (solid line) or 0.06 ug APC Armenian Hamster isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD80 Antibody [16-10A1]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;PE
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB50-F-25ug
CD80 General Information
Alternate Names
B7-1, B7.1
Curated Database and Bioinformatic Data
Gene SymbolCd80
Entrez Gene ID12519
Ensemble Gene IDENSMUSG00000075122
RefSeq Protein Accession(s)XP_017172346, NP_033985, XP_006521800, XP_017172347
RefSeq mRNA Accession(s)NM_009855, XM_017316857, XM_017316858, XM_006521737
RefSeq Genomic Accession(s)NC_000082
UniProt ID(s)Q00609, Q549R2
UniGene ID(s)Q00609, Q549R2
Cosmic ID(s)Cd80
KEGG Gene ID(s)mmu:12519
General Description of CD80.
The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Mouse Anti-CD80 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD80 Antibody Clone: 16-10A1
Clone 16-10A1 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD80 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD80 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD80 could be seen in. We were unable to detect CD80 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (in vitro blocking, Flow cytometry)Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry - OCT embedded frozen tissue)Anraku M, Tagawa T, Wu Licun, Yun Z, Keshavjee S, Zhang L, Johnston MR, and de Perrot M. 2010. J. Immunol. 185:956-966. (Flow cytometry) Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy and Immunohistochemistry - frozen tissue)Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N, Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking)Razi-Wold Z, Freeman GJ, Galvin F, Benacerraf B, Nadler L, and Reiser H. 1992. Proc. Natl. Acad. Sci. 89:4210-4214. (Origination of clone, Immunoprecipitation, in vitro blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD80 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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