C57Bl/6 bone marrow cells were stained with 0.5 ug APC Mouse Anti-Ly-6G (QAB56) (solid line) or 0.5 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-Ly-6G Antibody [1A8]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB56-F-25ug
Ly-6G General Information
Alternate Names
Curated Database and Bioinformatic Data
Gene SymbolLy6g
Entrez Gene ID546644
Ensemble Gene IDENSMUSG00000022582
RefSeq Protein Accession(s)NP_001297367, XP_006521677
RefSeq mRNA Accession(s)NM_008531, NM_001310438, XM_006521614
RefSeq Genomic Accession(s)NC_000081,
UniProt ID(s)A0A087WQF5
Cosmic ID(s)Ly6g
KEGG Gene ID(s)mmu:546644
General Description of Ly-6G.
The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

Mouse Anti-Ly-6G Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Ly-6G Antibody Clone: 1A8
Clone 1A8 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Ly-6G Previously Observed Antibody Staining Patterns

Selected References

Kamaly N, Fredman G, Subramanian M, Gadde S, Pesic A, Cheung L, Fayad ZA, Langer R, Tabas I and Cameron O. 2013. Proc. Natl. Acad. Sci. 110. 6506-6511. (flow cytometry). Stinn W, Buettner A, Weiler H, Friedrichs B, Luetjen S, van Overveld F, Meurrens K, Janssens K, Gebel S, Stabbert R and Haussman H-J. 2013. Toxicol. Sci. 131: 596-611 (flow cytometry).Enoksson M, Moller-Westerberg C, Wicher G, Fallon PG, Forsberg-Nilsson K, Lunderius-Andersson C and Nilsson G. 2013. Blood. 121. 530-536 (flow cytometry).

Limitations and Warranty

enQuire Bio's Mouse Anti-Ly-6G Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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