C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II APC (QAB70) (solid line) or 0.06 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-MHC Class II Antibody (I-A/I-E) [M5/114.15.2]

$ 49.00$ 379.00
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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB70-F-25ug
MHC class II antigen General Information
Alternate Names
histocompatibility 2, class II antigen A, beta 1, histocompatibility 2, class II antigen E, beta, H2Ab1, H2Eb1, H2-Ab1, H2-Eb1, MHC II
Curated Database and Bioinformatic Data
Gene SymbolH2-Ab1 and H2-Eb1
Entrez Gene ID14961, 14969
Ensemble Gene IDENSMUSG00000073421, ENSMUSG00000060586
RefSeq Protein Accession(s)NP_996988, NP_034512
RefSeq mRNA Accession(s)NM_207105, NM_010382
RefSeq Genomic Accession(s)NG_187027, NC_000083, NG_187004, NG_039662NG_187004, NC_000083, NG_187027, NG_039662
UniProt ID(s)Q3TD53, P14483, Q8BPA0, O78196
UniGene ID(s)Q3TD53, P14483, Q8BPA0, O78196
Cosmic ID(s)H2-Ab1, H2-Eb1
KEGG Gene ID(s)mmu:14961, mmu:14969
General Description of MHC class II antigen.
The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Mouse Anti-MHC class II antigen Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-MHC class II antigen Antibody Clone: M5/114.15.2
Clone M5/114.15.2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

MHC class II antigen Previously Observed Antibody Staining Patterns

Selected References

Staehli F, Ludigs K, Heinz LX, Segin-Estevez Q, Ferrero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. (Flow cytometry)Parra D, Rieger AM, Li J, Zhang Y-A, Randall LM, Hunter CA, Barreda DR, and Sunyer JO. 2012. J. Leukoc. Biol. 91:525-536. (in vitro blocking, Flow cytometry)Scarlett UK, Rutkowski MR, Rauwerdink AM, Fields J, Escovar-Fadul X, Baird J, Cubillos-Ruiz JR, Jacobs AC, Gonzalez JL, Weaver J, Fiering S, and Conejo-Garcia JR. 2012. J. Exp. Med. 209: 495-506. (Immunofluorescence microscopy - frozen tissue)Chen M, Felix K, and Wang J. 2011. J. Immunol. 187: 5684-5692. (in vitro blocking)Busman-Sahay K, Sargent E, Harton JA, and Drake JR. 2011. J. Immunol. 186:6710-6717. (Immunoprecipitation)Ohmura-Hoshino M, Matsuki Y, Aoki M, Goto E, Mito M, Uematsu M, Hakiuchi T, Hotta H, and Ishido S. 2006. J. Immunol. 177:341-354. (Immunofluorescence microscopy - frozen tissue, Immunoprecipitation)Li C, Siemasko K, Clark MR, and Song W. 2002. Int. Immunol. 14: 1179-1191. (Western Blot, Immunoelectron microscopy)

Limitations and Warranty

enQuire Bio's Mouse Anti-MHC Class II Monoclonal (I-A/I-E) is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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