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Recombinant Human PCNA Protein

$ 178.00$ 448.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP6470
Species: Human
Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Available Tags: His
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 261

Product NumberDescriptionPrice
QP6470-ec-10ug Size: 10 ug, Tag: His $ 158.00
QP6470-ec-100ug Size: 100 ug, Tag: His $ 398.00
Shipping Information
2-4 weeks
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP6470-ec-10ug
Recombinant Human PCNA Protein General Information
Alternate Names
PCNA, ATLD2
Curated Database and Bioinformatic Data
Gene SymbolPCNA
Entrez Gene ID5111
Ensemble Gene IDENSG00000132646
RefSeq Protein Accession(s)NP_002583.1
RefSeq mRNA Accession(s)NM_002592.2, NM_182649.1
UniProt ID(s)P12004
UniGene ID(s)Hs.147433
HGNC ID(s)HGNC:8729
COSMIC ID Link(s)PCNA
KEGG Gene ID(s)hsa:5111
PharmGKB ID(s)PA263
General Description of Recombinant Human PCNA Protein.
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Human PCNA Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant PCNA based upon sequence from Human
Host: QP6470 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) PCNA, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6470.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 - Ser261
Amino Acid Sequence: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human PCNA Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human PCNA Protein