SEQUEST analysis of LC MS/MS spectra obtained from a run with QP6719 identified a match between this protein and the spectra of a peptide sequence that matches a region of Hyaluronidase PH-20.
SEQUEST analysis of LC MS/MS spectra obtained from a run with QP6719 identified a match between this protein and the spectra of a peptide sequence that matches a region of Hyaluronidase PH-20.Additional SEQUEST analysis of the LC MS/MS spectra from QP6719 identified an additional between this protein and the spectra of another peptide sequence that matches a region of Hyaluronidase PH-20 confirming successful recombinant synthesis.

Recombinant Human Hyaluronidase PH-20 Protein

$ 148.00$ 1,678.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP6719
Species: Human
Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Available Tags: His-SUMO
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 455

Size
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Shipping Information
1-2 weeks
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP6719-ec-10ug
Recombinant Human Hyaluronidase PH-20 Protein General Information
Alternate Names
HEL-S-96n, HYAL1, SPAG15, HYAL5, PH20, HYA1, HYAL3, PH-20
Curated Database and Bioinformatic Data
Gene SymbolSPAM1
Entrez Gene ID6677
Ensemble Gene IDENSG00000106304
RefSeq Protein Accession(s)NP_001167515.1
RefSeq mRNA Accession(s)NM_001174044.1, NM_001174045.1, NM_001174046.1, NM_003117.4, NM_153189.2
UniProt ID(s)P38567
UniGene ID(s)Hs.121494
HGNC ID(s)HGNC:11217
COSMIC ID Link(s)SPAM1
KEGG Gene ID(s)hsa:6677
PharmGKB ID(s)PA36053
General Description of Recombinant Human Hyaluronidase PH-20 Protein.
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are bedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Human Hyaluronidase PH-20 Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant Hyaluronidase PH-20 based upon sequence from Human
Host: QP6719 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Hyaluronidase PH-20, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6719.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Leu36 - Ser490
Amino Acid Sequence: LNFRAPPVIP NVPFLWAWNA PSEFCLGKFD EPLDMSLFSF IGSPRINATG QGVTIFYVDR LGYYPYIDSI TGVTVNGGIP QKISLQDHLD KAKKDITFYM PVDNLGMAVI DWEEWRPTWA RNWKPKDVYK NRSIELVQQQ NVQLSLTEAT EKAKQEFEKA GKDFLVETIK LGKLLRPNHL WGYYLFPDCY NHHYKKPGYN GSCFNVEIKR NDDLSWLWNE STALYPSIYL NTQQSPVAAT LYVRNRVREA IRVSKIPDAK SPLPVFAYTR IVFTDQVLKF LSQDELVYTF GETVALGASG IVIWGTLSIM RSMKSCLLLD NYMETILNPY IINVTLAAKM CSQVLCQEQG VCIRKNWNSS DYLHLNPDNF AIQLEKGGKF TVRGKPTLED LEQFSEKFYC SCYSTLSCKE KADVKDTDAV DVCIADGVCI DAFLKPPMET EEPQIFYNAS PSTLS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Hyaluronidase PH-20 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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