SDS-PAGE separation of QP7316 followed by commassie total protein stain results in a primary band consistent with reported data for Elongation factor P. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant E. coli Elongation factor P Protein

$ 728.00 – $ 2,958.00

Please Select Product Options Below To View The Catalog Number.

SKU: QP7316
Species: E. coli
Available Tags: His, His-SUMO
Host: E.coli, Yeast.
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 187

Product Number	Description	Price
QP7316-ye-100ug	Size: 100 ug, Tag: His	$ 828.00
QP7316-ye-1mg	Size: 1 mg, Tag: His	$ 2,958.00
QP7316-ec-100ug	Size: 100 ug, Tag: His-SUMO	$ 728.00
QP7316-ec-1mg	Size: 1 mg, Tag: His-SUMO	$ 2,468.00

Shipping Information

On Demand Protein: 3-5 Week Lead Time. (Why?)

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QP7316-ye-10ug

Recombinant E. coli Elongation factor P Protein General Information
Alternate Names
ECK4141; JW4107; b4147
Curated Database and Bioinformatic Data
Gene Symbol	efp
Entrez Gene ID	948661
RefSeq Protein Accession(s)	NP_418571.1
RefSeq mRNA Accession(s)	NC_000913.3, NZ_LN832404.1
UniProt ID(s)	P0A6N4
KEGG Gene ID(s)	ecj:JW4107
General Description of Recombinant E. coli Elongation factor P Protein.
Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis.

E. coli Elongation factor P Recombinant Protein Product Attributes

Product Type: Recombinant Protein

Recombinant Elongation factor P based upon sequence from: E. coli

Host: QP7316 protein expressed in E.coli, Yeast..

Tag: His

Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Elongation factor P, was expressed in the hosts and tags indicated. Please select your host/tag option, above.

Application Notes: Please contact us for application specific information for QP7316.

Bioactivity Data: Untested

Full Length? Full Length

Expression Region: Ala2 - Lys188

Amino Acid Sequence: ATYYSNDFRA GLKIMLDGEP YAVEASEFVK PGKGQAFARV KLRRLLTGTR VEKTFKSTDS AEGADVVDMN LTYLYNDGEF WHFMNNETFE QLSADAKAIG DNAKWLLDQA ECIVTLWNGQ PISVTPPNFV ELEIVDTDPG LKGDTAGTGG KPATLSTGAV VKVPLFVQIG EVIKVDTRSG EYVSRVK

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution Instructions:

Concentration of E. coli Elongation factor P Protein:

Endotoxin Levels: Not determined.

Buffer: Tris-based buffer, 50% glycerol

Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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