Western blot data demonstrating successful knockdown of Estrogen Receptor (ER) by QX16 at 48 hrs post transfection (TC = Transfection Control, SC = Scrambled Control (Product Number QC1), siRNA = QX16 treatment)
Western blot data demonstrating successful knockdown of Estrogen Receptor (ER) by QX16 at 48 hrs post transfection (TC = Transfection Control, SC = Scrambled Control (Product Number QC1), siRNA = QX16 treatment)

TranslationBlocker Human Estrogen receptor beta siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Human Estrogen receptor beta siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX16-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 219.00
QX16-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 329.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QX16-2nmol

Human Estrogen receptor beta TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of Estrogen receptor beta from: Human.
May also react with Estrogen receptor beta based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_001040276.
Samples and cell lines tested: MCF7 Human Adenocarcinoma Cells from Breast Cancer.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human Estrogen receptor beta: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    Estrogen receptor beta Knockdown Protocol:
    1. Starting with cultured cells in growth media seed 6 well plates with 1x105 cells and incubate at 37°C for until cells reach 40-50% confluence.
    2. Prepare transfection reagent in serum-free growth media such as Opti-MEM-I. Allow to come to room temperature. The above data was generated with Oligofectamine for delivery of Estrogen Receptor Beta (ERBeta) siRNA prepared in accordance with manufacturer’s instructions.
    3. Dilute siRNA to working concentration in serum-free growth media. For Oligofectamine or similar transfections into 6 well plates, we recommend starting siRNA concentration optimization at around 200 pmol siRNA duplex in 200 ul of serum free media.Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared Oligofectamine or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 30 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells. For 6 well plates this will entail adding the solution to cells in approximately 2.5 ml serum-free media. Additionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    6. Incubate for several hours in serum free media.
    7. Allow cells to recover by adding serum containing media to culture without a media change. Generally 1-2 fold of the original volume of siRNA containing serum free media is added to restore nutrient levels in media.
    8. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. Estrogen Receptor Beta (ERBeta) knockdown at the protein level was measured at 48 hours post transfection via western blot.

Estrogen receptor beta General Information

Gene Name: ESR2
Entrez Gene ID: 13983
NCBI Ref Seq Accession Number: NP_034287
Uniprot ID:
Estrogen receptor beta Molecular Weight:
Alternate Names: ESR-BETA, NR3A2, ESTRB, Erb, ESRB, ER-BETA, ERBeta

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “TranslationBlocker Human Estrogen receptor beta siRNA”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

TranslationBlocker Human Estrogen receptor beta siRNA

https://enquirebio.com/wp-content/uploads/2017/07/QX16-2nmol-Estrogen-receptor-beta-RNAi-data.jpg