Flow Cytometry data demonstrating successful knockdown of ErbB4 / Her4 72 hours after treatment with QX21 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX21 treatment)
Flow Cytometry data demonstrating successful knockdown of ErbB4 / Her4 72 hours after treatment with QX21 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX21 treatment)

TranslationBlocker Human ErbB4 / Her4 siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Human ErbB4 / Her4 siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX21-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 219.00
QX21-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 329.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QX21-2nmol

Human ErbB4 / Her4 TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of ErbB4 / Her4 from: Human.
May also react with ErbB4 / Her4 based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_005235.
Samples and cell lines tested: T-47d Human Carcinoma Cells from Breast.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human ErbB4 / Her4: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    ErbB4 / Her4 Knockdown Protocol:
    1. Starting with cultured cells in growth media seed 12 well plates with 5x104 cells and incubate at 37°C.
    2. Prepare transfection reagent in serum-free growth medium such as Opti-MEM-I. Allow to come to room temperature. Oligofectamine was used to generate the above data in accordance with manufacturer’s instructions.
    3. Dilute siRNA to working concentration in serum-free growth media. For Oligofectamine or similar transfections into 12 well plates, we recommend starting siRNA concentration optimization at around 100-150 pmol siRNA duplex in 200 ul of serum free media.Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared Oligofectamine or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 30 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells. For 12 well plates this will entail adding the solution to cells in approximately 1.1 ml serum-free medium. Additionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    6. Incubate for several hours in serum free media. The above data was generated with an initial 4 hour incubation in serum-free media.
    7. Add serum containing media to culture without a media change. Generally 1-2 fold of the original volume of siRNA containing serum-free media is added to restore nutrient levels in media. Alternatively, the media may be directly supplemented with serum such as 10% FBS.
    8. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. ErbB4 / Her4 knockdown at the protein level was measured at 72 hours post transfection via western blot.

ErbB4 / Her4 General Information

Gene Name: ERBB4
Entrez Gene ID: 2066
NCBI Ref Seq Accession Number: NP_005226
Uniprot ID:
ErbB4 / Her4 Molecular Weight:
Alternate Names: p180erbB4, HER4, ALS19, Erb-b4, ErbB-4

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “TranslationBlocker Human ErbB4 / Her4 siRNA”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

TranslationBlocker Human ErbB4 / Her4 siRNA

https://enquirebio.com/wp-content/uploads/2017/07/QX21-2nmol-ErbB4-Her4-RNAi-data.jpg