ELISA data demonstrating successful knockdown of TNF alpha 26 hours after treatment with QX22 siRNA (Control = Scrambled Control (Product Number QC1), siRNA = QX22 treatment)
ELISA data demonstrating successful knockdown of TNF alpha 26 hours after treatment with QX22 siRNA (Control = Scrambled Control (Product Number QC1), siRNA = QX22 treatment)

TranslationBlocker Human TNF alpha siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Human TNF alpha siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX22-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 219.00
QX22-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 329.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QX22-2nmol

Human TNF alpha TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of TNF alpha from: Human.
May also react with TNF alpha based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: BC028148.
Samples and cell lines tested: Adherent LPS Treated Human PBMCs and Adherent dsRNA Treated Human PBMCs.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human TNF alpha: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    TNF alpha Knockdown Protocol:
    1. Note: siRNAs may stimulate inflammatory processes and care must be taken when using these as tools to study the inflammatory response. This response is not present for all siRNAs, but is sequence specific. This TNF alpha specific siRNA has been tested and shown to have minimal impact on the inflammatory response in human adherent PBMCs. However, additional testing should be undertaken to ensure appropriateness for your experiments.

    2. Starting with cultured cells in growth media seed plates with cells. For the above data, 5x104 cells were seeded into 96 well plates and incubated at 37°C.
    3. Prepare transfection reagent in serum-free growth medium such as Opti-MEM-I. Allow to come to room temperature. DOTAP was used to generate the above data in accordance with manufacturer’s instructions. It is a cationic liposome similar to Lipofectamine 2000 or other transfection reagents.
    4. Dilute siRNA to working concentration in serum-free growth media. For DOTAP or similar transfections into 96 well plates, we recommend starting siRNA dose optimization at around 20 pmol siRNA duplex.Optimal dilution should be experimentally determined.
    5. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared DOTAP or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 30 minutes with gentle mixing (optional).
    6. Add prepared siRNA solution to cells. For 96 well plates this will entail adding the solution to cells in approximately 0.2 ml serum-free medium. For calculating scale up, 12 well plates will require nearer to 1.2 ml of media. Optionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    7. Incubate for several hours in serum free media. The above data was generated with an 18 hour incubation.
    8. Add serum containing media to culture without a media change if longer incubation times are required. Generally 1-2 fold of the original volume of siRNA containing serum-free media is added to restore nutrient levels in media. Alternatively, the media may be directly supplemented with serum such as 10% FBS.
    9. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. TNF alpha knockdown at the protein level was measured at 26 hours post transfection via ELISA. Stimulation with 20ng/ml LPS to induce a TNF alpha response began 18 hours post siRNA treatment and lasted 8 hours.

TNF alpha General Information

Gene Name: TNF
Entrez Gene ID: 7124
NCBI Ref Seq Accession Number: NP_000585
Uniprot ID:
TNF alpha Molecular Weight:
Alternate Names: DIF, TNFA, TNFSF2, TNLG1F, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “TranslationBlocker Human TNF alpha siRNA”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

TranslationBlocker Human TNF alpha siRNA

https://enquirebio.com/wp-content/uploads/2017/07/QX22-2nmol-TNF-alpha-RNAi-data-300x182.jpg