Western Blot data demonstrating successful knockdown of TLR8 in human cells 48 hours after treatment with QX27 siRNA (TC = Transfection Control, siRNA = QX27 treatment)
Western Blot data demonstrating successful knockdown of TLR8 in human cells 48 hours after treatment with QX27 siRNA (TC = Transfection Control, siRNA = QX27 treatment)

TranslationBlocker Human TLR8 / CD288 siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Human TLR8 / CD288 siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX27-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 219.00
QX27-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 329.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Bioactivity (test results eg. IU/ml):


SKU: QX27-2nmol

Human TLR8 / CD288 TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of TLR8 / CD288 from: Human.
May also react with TLR8 / CD288 based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_016610.
Samples and cell lines tested: 293T Human Embryonic Kidney Cells with Large T Antigen.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human TLR8 / CD288: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    TLR8 / CD288 Knockdown Protocol:
    1. Starting with cultured cells in growth media seed plates with cells and incubated at 37°C. The above data was generated in 6 well plates with 1.2x106 cells.
    2. Prepare transfection reagent in serum-free growth medium such as Opti-MEM-I. Allow to come to room temperature. Lipofectamine 2000 was used to generate the above data in accordance with manufacturer’s instructions. li>
    3. Dilute siRNA to working concentration in serum-free growth media. For Lipofectamine or similar transfections into 6 well plates, we recommend starting siRNA dose optimization at around 150 pmol siRNA duplex.Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared Lipofectamine 2000 or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 30 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells. For 6 well plates this will entail adding the solution to cells in approximately 2.5 ml serum-free medium. Optionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    6. Incubate for several hours in serum free media. The above data was generated with a 4 hour incubation.
    7. Add serum containing media to culture without a media change if longer incubation times are required. Generally 1-2 fold of the original volume of siRNA containing serum-free media is added to restore nutrient levels in media. Alternatively, the media may be directly supplemented with serum such as 10% FBS.
    8. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. TLR8 knockdown at the protein level was measured at 48 hours post transfection via Western Blot.

TLR8 / CD288 General Information

Gene Name: TLR8
Entrez Gene ID: 51311
NCBI Ref Seq Accession Number: NP_057694
Uniprot ID:
TLR8 / CD288 Molecular Weight:
Alternate Names: CD288, TLR8, Toll-like receptor 8, TLR-8

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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TranslationBlocker Human TLR8 / CD288 siRNA

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