Western blot data demonstrating successful knockdown of Caspase-2 in doxorubicin treated human cells 48 hours after treatment with QX39 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX39 treatment)
Western blot data demonstrating successful knockdown of Caspase-2 in doxorubicin treated human cells 48 hours after treatment with QX39 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX39 treatment)

TranslationBlocker Human Caspase-2 siRNA

$ 248.00$ 368.00
Please Select Product Options Below To View The Catalog Number.

Human Caspase-2 siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX39-2nmol : 2-nmol: untagged $ 248.00
QX39-10nmol : 10-nmol: untagged $ 368.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QX39-2nmol

Human Caspase-2 TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of Caspase-2 from: Human.
May also react with Caspase-2 based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_032983.
Samples and cell lines tested: U-2 OS Human Osteosarcoma Epithelial Cells from Bone.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human Caspase-2: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    Caspase-2 Knockdown Protocol:
    1. Starting with cultured cells in growth media seed plates with cells and incubated at 37°C. For the above data 6 well plates were seeded with 1x105 cells and incubated at 37°C for 24 hours.
    2. Prepare transfection reagent in serum-free growth medium such as Opti-MEM-I. Allow to come to room temperature. Oligofectamine was used to generate the above data in accordance with manufacturer’s instructions. li>
    3. Dilute siRNA to working concentration in serum-free growth media. For Oligofectamine or similar transfections into 12 well plates, we recommend starting siRNA dose optimization at around 100-125 pmol siRNA duplex.Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Ratio of transfection reagent to siRNA should be optimized. Equivalent volumes of prepared Oligofectamine or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 30 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells. For 6 well plates this will entail adding the solution to cells in approximately 1.1 ml serum-free medium. Optionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    6. Incubate for several hours in serum free media. Typically, 4 hour incubation is recommended for this initial transfection step.
    7. Add serum containing media to culture without a media change if longer incubation times are required. Generally 1-2 fold of the original volume of siRNA containing serum-free media is added to restore nutrient levels in media. Alternatively, the media may be directly supplemented with serum such as 10% FBS.
    8. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. Caspase-2 knockdown at the protein level was measured at 48 hours post transfection via Western Blot.

Caspase-2 General Information

Gene Name: CASP2
Entrez Gene ID: 835
NCBI Ref Seq Accession Number: NP_116765
Uniprot ID:
Caspase-2 Molecular Weight:
Alternate Names: CASP-2, ICH1, PPP1R57, NEDD-2, NEDD2, CASP2

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “TranslationBlocker Human Caspase-2 siRNA”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

TranslationBlocker Human Caspase-2 siRNA

https://enquirebio.com/wp-content/uploads/2017/07/QX39-2nmol-Caspase-2-RNAi-data.jpg