Western blot data demonstrating successful knockdown of BAX by QX9 at 48 hrs post transfection (SC = Scrambled Control (Product Number QC1), siRNA = QX9 treatment)
Western blot data demonstrating successful knockdown of BAX by QX9 at 48 hrs post transfection (SC = Scrambled Control (Product Number QC1), siRNA = QX9 treatment)

TranslationBlocker Mouse Apoptosis regulator BAX siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Mouse Apoptosis regulator BAX siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX9-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 329.00
QX9-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 219.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QX9-10nmol

Mouse Apoptosis regulator BAX TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of Apoptosis regulator BAX from: Mouse.
May also react with Apoptosis regulator BAX based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_007527.
Samples and cell lines tested: DC-1 Mouse Dendritic Cells.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Mouse Apoptosis regulator BAX: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    Apoptosis regulator BAX Knockdown Protocol:
    1. Starting with cultured cells in growth media seed 6 well plates with cells and incubate at 37°C until cells reach 40% confluence. In the example data, larger cultures of 2 x 105 were transfected
    2. Prepare transfection reagent in serum-free growth media such as Opti-MEM allow to come to room temperature. The above data was generated with Oligofectamine for delivery of mouse BAX siRNA prepared in accordance with manufacturer’s instructions.
    3. Dilute siRNA to working concentration in serum-free growth media. For Oligofectamine or similar transfections into 6 well plates, we recommend starting siRNA concentration optimization at around 150 pmol siRNA duplex in 300 ul of serum free media. Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared Oligofectamine or other transfection reagent and diluted siRNA should be combined and incubated at room temperature for 20 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells. Additionally, FITC labeled control siRNA (Product Number QC-1F) or positive control siRNA (Beta-Actin; Product Number QC-2) may be used to assess transfection efficiency.
    6. Incubate for several hours in serum free media.
    7. Add serum containing media to culture without a media change. Generally 1-2 fold of the original volume of siRNA containing serum free media is added to restore nutrient levels in media.
    8. Incubate and assay several hours after transfection. Optimal knockdown time should also be experimentally determined. Optionally, a second transfection can be performed 24 hours after the first transfection to enhance knockdown. Mouse BAX knockdown at the protein level was measured at 48 hours post transfection via western blot.

Apoptosis regulator BAX General Information

Gene Name: Bax
Entrez Gene ID: 12028
NCBI Ref Seq Accession Number: NP_031553
Uniprot ID:
Apoptosis regulator BAX Molecular Weight:
Alternate Names: BCL2L4

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “TranslationBlocker Mouse Apoptosis regulator BAX siRNA”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

TranslationBlocker Mouse Apoptosis regulator BAX siRNA

https://enquirebio.com/wp-content/uploads/2017/07/QX9-10nmol-Apoptosis-regulator-BAX-RNAi-data.jpg