100 ul of predilute SingleStep™ Poly-HRP conjugated goat anti-rabbit secondary antibody was incubated 30 minutes following anti-EpCAM staining of human rectum samples.  Excellent signal intensity is observed in the absence of any additional amplification step.
100 ul of predilute SingleStep™ Poly-HRP conjugated goat anti-rabbit secondary antibody was incubated 30 minutes following anti-EpCAM staining of human rectum samples.  Excellent signal intensity is observed in the absence of any additional amplification step.200 ul of predilute SingleStep™ Poly-HRP conjugated goat anti-rabbit secondary antibody was incubated 30 minutes following anti-NKX2-2 staining of human pancreas.  Excellent signal intensity is observed in the absence of any additional amplification step.

SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody

$ 59.00$ 599.00
Please Select Product Options Below To View The Catalog Number.

SKU: Q2AB2
Species: Rabbit
Tested Applications: Immunohistochemistry
Available Conjugates: SingleStep™ Poly-HRP
Isotype: Goat IgG
Mass Spec Validated?: Not MS Validated

Format:
Size Clear selection
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: Q2AB2-5ml

Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody Antibody Product Attributes

This poly-HRP secondary antibody has been tested to react with Rabbit primary antibodies in immunohistochemistry (IHC).
Does not cross-react with human, bovine, or equine. Other species not tested.
Tested Applications: Immunohistochemistry.
Application Notes: If additional reduction of non-specific signal is required, blocking with 5-10% Normal Goat Serum (NGS) is recommended.
Clonality: Polyclonal
Host: Goat
Buffer and Stabilizer: Pre-dilute antibody is in proprietary signal enhancement buffer composed of protein based blocking agent (non-BSA), non-ionic detergent, preservative (non-azide), and HRP-stabilizer at pH 7.4. Concentrated format also contains 50% glycerol.
Antibody Concentration: Prediluted format is at 100-400 ul per IHC test. Concentrated format is at 1 mg/ml.
Antibody Purification Method:Antibody is initially purified by ammonium sulfate precipitation followed by cross-adsorption to ensure minimal cross-reactivity to human, bovine, and equine antibodies. Purification of SingleStep™ poly-HRP conjugated goat anti-rabbit secondary is achieved via size exclusion liquid chromatography.
Immunogen: Generation of this anti-Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody antibody was achieved by immunizing a Goat host with Pooled population of Rabbit IgG
Storage Conditions: Store at prediluted poly-HRP conjugated secondary antibody at 4 C and concentrated format at -20C. Do not freeze prediluted antibody. Stable for >1 year if stored properly.
Frequently Used Alternate Names: Poly-HRP Conjugated Goat Anti-Rabbit Whole IgG Secondary, HRP Polymer Conjugated Goat Anti-Rabbit Whole IgG Secondary(Additional alternate names exist but are infrequently used and possibly ambiguous).
General Information for Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody: SingleStep™ Poly-HRP (Horse Radish Peroxidase) conjugated goat anti-rabbit secondary antibodies provide signal amplification over traditional HRP conjugated reagents in immunohistochemistry (IHC) staining using primary antibodies from a rabbit host. The signal amplification afforded by these HRP polymer conjugated antibodies is sufficient to enable application of a single secondary antibody unlike standard HRP conjugated reagents which utilize an unconjugated or biotinylated secondary antibody and then an additional HRP conjugated secondary antibody or HRP conjugted streptavidin to visualize staining. These additional staining steps increase background staining and variability between samples which is obviously undesirable in IHC. SingleStep™ goat anti-rabbit poly-HRP secondary antibodies are sourced from the industry leading secondary antibody manufacturer (you're probably already using these in your lab) and are then conjugated to a carefully controlled number of HRP molecules to facilitate excellent tissue penetration without sacrificing signal amplification. Our predilute products also incorporate our enhanced IHC staining buffer which contains optimized stabilizers and blocking reagents to ensure maximum sensitivity and high reproducibility in IHC in most sample types.

Limitations and Warranty

enQuire Bio's Epcam / CD326 Anti-Rat, Rabbit Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-Q2AB2-5ml-anti-Poly-HRP-Anti-Rabbit-IgG-HL-Secondary-Antibody-antibody-300x225.jpeghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-Q2AB2-5ml-anti-Poly-HRP-Anti-Rabbit-IgG-HL-Secondary-Antibody-antibody-1-300x225.jpeg