Brand

Why?…   It’s the question at the heart of what it means to be a scientist. It is our search for answers that leads us down the road to discovery. Is SDS-PAGE enough to validate recombinant proteins? Is my antibody specific? Will my siRNA knock down levels of the protein? When we discover one answer,...

Support

Technical Support Need help immediately? Email Technical Support FAQs Do you have a protocol for my protein or antibody? General protocols, which are the basis for our product validation, are available by application. We primarily sell antibodies for use in flow cytometry, immunohistochemistry, and immunocytochemistry/icc. We are working to provide protocols that are specific to...

ICC/IF Protocol

Immunocytochemistry (ICC) is a common research technique by which a user is able to view and identify cellular components and the locations of proteins of interest in intact cells. Typically, ICC is performed using fluorescently labeled antibodies (Immunofluorescence – IF) for easier multiplexing which facilitates visualization of colocalization of proteins. However, ICC can also be performed...

IHC Antibodies Details

What Sets Our IHC Antibodies Apart Our monoclonal antibodies for immunohistochemistry (IHC) are sourced from the best in the business: NeoBiotechnologies. Although you may not be familiar with NeoBiotechnologies, we will outline what sets them apart from other companies and why we chose them as a partner for our IHC monoclonal antibodies. We believe you will...

Validation Project

Our Mission: Better, truly quantitative antibody validation. Click here for details on our Mass Spec MS-QAVA™ Validation Methods and to see our MS-QAVA™ mAbs. MS-QAVA™: Mass Spec Based Quantitative Antibody Validation.   Our products will take the guess work out of determining antibody specificity with a novel Mass-Spec based validation method. Click to learn more. Even CRISPR validation only...

Validation Project Details

MS QAVA™:  Quantitative Measurement of Antibody Specificity Powered by Mass Spec. Existing validation only provides approximations and assumptions of specificity. Here’s how it works: Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared.  r-squared represents variability in...

Validation Project Details