Search Results for: protein

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Why?…   It’s the question at the heart of what it means to be a scientist. It is our search for answers that leads us down the road to discovery. Is SDS-PAGE enough to validate recombinant proteins? Is my antibody specific? Will my siRNA knock down levels of the protein? When we discover one answer,...

Technical Support Need help immediately? Email Technical Support FAQs Do you have a protocol for my protein or antibody? General protocols, which are the basis for our product validation, are available by application. We primarily sell antibodies for use in flow cytometry, immunohistochemistry, and immunocytochemistry/icc. We are working to provide protocols that are specific to...

Immunocytochemistry (ICC) is a common research technique by which a user is able to view and identify cellular components and the locations of proteins of interest in intact cells. Typically, ICC is performed using fluorescently labeled antibodies (Immunofluorescence – IF) for easier multiplexing which facilitates visualization of colocalization of proteins. However, ICC can also be performed...

One Final Tip: Use Highly Validated Antibodies!

MS QAVA™:  Quantitative Measurement of Antibody Specificity Powered by Mass Spec. Existing validation only provides approximations and assumptions of specificity. Here’s how it works: Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared.  r-squared represents variability in...