FFPE human lymph node sections stained with 100 ul anti-AIF1 (clone AIF1/1909) at 1:400. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-AIF1 (clone AIF1/1909) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Data from SDS-PAGE analysis of Anti-AIF1 antibody (Clone AIF1/1909). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.

Formalin-fixed, paraffin-embedded Human Lymph Node stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/1909).

AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

Name: AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody <p style="font-size:small">Tested against &gt;20,000 Human Proteins</p>
SKU: 199-MSM1-P0
Availability: InStock

$ 199.00 – $ 429.00

SKU: 199-MSM1
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Size

Tag

Clear

SKU: 199-MSM1-P0 Categories: Antibody, Human, IgG1, IHC, kappa, Mouse, Not MS Validated, Ultraspecific Antibodies, Unconjugated Tag: AIF1 / Iba1 (Microglia Marker)

PDF Datasheet

Human Anti-AIF1 / Iba1 (Microglia Marker) Antibody Product Attributes

Species: Human

Tested Applications: IHC.

Clonality: Monoclonal

Anti-AIF1 / Iba1 (Microglia Marker) Antibody Clone: AIF1/1909

Clone AIF1/1909 Host and Isotype: Mouse IgG1, kappa

Anti-Human AIF1 / Iba1 (Microglia Marker) Positive Control Sample: Lymph Node, Tonsil or Kidney

Cellular Localization of Antibody AIF1/1909 Staining: Cytoplasmic and Cell Surface

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.

Antibody Concentration: 200 ug/ml

Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.

Immunogen: Purified fragment of human recombinant AIF1 protein (apprx. aa 1-146) (Please call for additional information.)

Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

AIF1 / Iba1 (Microglia Marker) Previously Observed Antibody Staining Patterns

AIF1 / Iba1 (Microglia Marker) General Information
Alternate Names
Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
Molecular Weight
17kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol	199
Entrez Gene ID	AIF1
UniProt ID(s)	P55008
UniGene ID(s)	Hs76364
COSMIC ID Link(s)	AIF1
KEGG Gene ID(s)	hsa:199
General Description of AIF1 / Iba1 (Microglia Marker).
AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Limitations and Warranty

enQuire Bio’s product, AIF1 / Iba1 (Microglia Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

Size	20 ug, 100 ug
Tag	Unconjugated
Buffer and Stabilizer	10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Product Type	Antibody
Host	Mouse
Isotype	IgG1, kappa
Applications	IHC
Species	Human
Mass Spec Validated?	Not MS Validated

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AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody Tested against >20,000 Human Proteins

Human Anti-AIF1 / Iba1 (Microglia Marker) Antibody Product Attributes

AIF1 / Iba1 (Microglia Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

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