PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:75 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Actin, Muscle Information for Pathologists
Summary:
Discovered in 1987 (Am J Pathol 1987;126:51); also called HHF35, MSA. Recognizes all alpha actins (skeletal, smooth, cardiac) and gamma smooth muscle actin; but not beta cytoplasmic or gamma cytoplasmic actin (the latter is also called non-muscle actin). Recognizes actin expressed in all cells with muscle differentiation (cardiac, smooth and skeletal muscle), myoepithelial cells, myofibroblasts, pericytes and myogenic tumors (Am J Clin Pathol 1991;96:32). Uses by pathologists Identify skeletal muscle (Tumori 2007;93:198, J Cutan Pathol 2007;34:352) and smooth muscle cells (Eur Respir J 2001;17:316) in normal tissue or various disease entities.
Common Uses By Pathologists:
Identify skeletal muscle (Tumori 2007;93:198, J Cutan Pathol 2007;34:352) and smooth muscle cells (Eur Respir J 2001;17:316) in normal tissue or various disease entities. Classify tumors with smooth or skeletal muscle, pericytes, myofibroblasts (Cardiovasc Pathol 2006;15:91) or myoepithelial cells. Differentiate leiomyosarcoma (MSA+, keratin-) from spindle cell carcinoma (MSA-, keratin+, Am J Otolaryngol 2005;26:201).
| Actin, Muscle General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 42.1 kDa | |
| Chromosomal Location | |
| q42.13 [chr: 1] [chr_start: 229431245] [chr_end: 229434098] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ACTA1 |
| Entrez Gene ID | 58 |
| RefSeq Protein Accession(s) | NP_001091 |
| RefSeq mRNA Accession(s) | NM_001100; |
| RefSeq Genomic Accession(s) | NG_006672; NC_000001 |
| UniProt ID(s) | P68133 |
| PharmGKB ID(s) | PA24455 |
| KEGG Gene ID(s) | hsa:58 |
| Associated Diseases (KEGG IDs) | Nemaline myopathy 3 (NEM3) [MIM:161800]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. {ECO:0000269|PubMed:10508519, ECO:0000269|PubMed:11166164, ECO:0000269|PubMed:11333380, ECO:0000269|PubMed:15198992, ECO:0000269|PubMed:15236405, ECO:0000269|PubMed:15336687, ECO:0000269|PubMed:15520409, ECO:0000269|PubMed:16427282, ECO:0000269|PubMed:16945537, ECO:0000269|PubMed:17705262, ECO:0000269|PubMed:22442437, ECO:0000269|PubMed:23650303, ECO:0000269|PubMed:25938801}. The disease is caused by mutations affecting the gene represented in this entry.; Myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]: A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. {ECO:0000269|PubMed:10508519}. The disease is caused by mutations affecting the gene represented in this entry.; Myopathy, congenital, with fiber-type disproportion (CFTD) [MIM:255310]: A genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. {ECO:0000269|PubMed:15468086, ECO:0000269|PubMed:17387733}. The disease is caused by mutations affecting the gene represented in this entry.; Myopathy, scapulohumeroperoneal (SHPM) [MIM:616852]: An autosomal dominant muscular disorder characterized by progressive muscle weakness with initial scapulo-humeral-peroneal and distal distribution. Over time, muscle weakness progresses to proximal muscle groups. Clinical characteristics include scapular winging, mild lower facial weakness, foot drop due to foot eversion and dorsiflexion weakness, and selective muscle atrophy. Age at onset and disease progression are variable. {ECO:0000269|PubMed:25938801}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Actin, Muscle . | |
| This pan actin is useful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma. | |
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