PDF DatasheetHuman, Rabbit, Cat, Canine, Mouse, Rat, and Chicken Anti-Actin, Muscle Specific Antibody Product Attributes
Species: Human, Rabbit, Cat, Canine, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Actin, Muscle Specific Antibody Clone: HHF35
Clone HHF35 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rabbit, Cat, Canine, Mouse, Rat, and Chicken Actin, Muscle Specific Positive Control Sample: Muscle or sarcoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: SDS extract of human myocardium.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Actin, Muscle Specific Previously Observed Antibody Staining Patterns
| Actin, Muscle Specific General Information | |
|---|---|
| Alternate Names | |
| ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1, anti-ACTA antibody, anti-ACTA1 antibody, anti-ACTA2 antibody, anti-ACTC1 antibody, anti-Actin antibody, anti-ACTSA antibody, anti-Alpha-2 actin antibody, anti-alpha skeletal muscle antibody, anti-Alpha-actin-1 antibody, anti-Cardiac muscle alpha actin-1 antibody, anti-Skeletal muscle alpha actin-1 antibody | |
| Molecular Weight | |
| Multiple | |
| Chromosomal Location | |
| 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth) |
| Entrez Gene ID | 58, 59, 70 |
| Ensemble Gene ID | ENSG00000143632, ENSG00000107796, ENSG00000159251 |
| RefSeq Protein Accession(s) | NP_001091, NP_001135417, NP_001604, NP_001307784, NP_005150 |
| RefSeq mRNA Accession(s) | NM_001100, NM_001141945 NM_001613, NM_001320855NM_005159 |
| RefSeq Genomic Accession(s) | NG_006672, NC_018912, NC_000001, NC_000010, NC_018921, NG_011541, NG_007553, NC_018926, NC_000015 |
| UniProt ID(s) | P68133, P02568, P99020, P03996, P04108, D2JYH4, P62736, P68032, B3KPP5 |
| UniGene ID(s) | P68133, P02568, P99020, P03996, P04108, D2JYH4, P62736, P68032, B3KPP5 |
| HGNC ID(s) | 129, 130, 143 |
| Cosmic ID(s) | ACTA1, ACTA2, ACTC1 |
| KEGG Gene ID(s) | hsa:58, hsa:59, hsa:70 |
| PharmGKB ID(s) | PA24455, PA24456, PA162375571 |
| General Description of Actin, Muscle Specific. | |
| This antibody recognizes actin of skeletal, cardiac,, smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta,, gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha, gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells, connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas,, lymphomas are negative.It stains tumors of smooth muscle (leiomyomas, leiomyosarcomas) as well as skeletal muscle (rhabdomyomas, rhabdomyosarcomas). | |
Limitations and Warranty
enQuire Bio’s Actin, Muscle Specific Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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