![Analysis of Mass Spec data (dashed-line) of fractions stained with Alkaline Phosphatase / PLAP MS-QAVA™ monoclonal antibody [Clone: GM022] (solid-line), reveals that less than 5.7% of signal is attributable to non-specific binding of anti-Alkaline Phosphatase / PLAP [Clone GM022] to targets other than ALPP protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-250-MSM4-P1-anti-Alkaline-Phosphatase-PLAP-antibody.png "Alkaline Phosphatase / PLAP Antibody Mass Spec Validation Data")
PDF DatasheetHuman Anti-Alkaline Phosphatase / PLAP Antibody Product Attributes
Alkaline Phosphatase / PLAP Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Variations in Placental Alkaline Phosphatase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of Placental Alkaline Phosphatase could be seen inglandular cells in the cervix, uterine, squamous epithelial cells in the cervix and uterine. We were unable to detect Placental Alkaline Phosphatase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Type:
Tissues from cancer patients, for instance, have their own distinct pattern of Placental Alkaline Phosphatase expression as measured by anti-Placental Alkaline Phosphatase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | + | – | – | – | – | – | – | + | – | – | – | – | – | +++ | – | – |
| ALPP Variability | + | + | ++ | + | ++ | + | + | + | + | + | + | ++ | + | + | + | + | ++ | + | + | + |
| Alkaline Phosphatase / PLAP General Information | |
|---|---|
| Alternate Names | |
| Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1, anti-Alkaline phosphatase placental type antibody, anti-Alkaline phosphatase Regan Isozyme antibody, anti-ALP antibody, anti-Alp1 antibody, anti-ALPP antibody, anti-Germ-cell alkaline phosphatase antibody, anti-nagao Isozyme antibody, anti-PALP antibody, anti-Placental alkaline phosphatase 1 antibody, anti-placental heat-stable alkaline phosphatase antibody, anti-PLAP-1 antibody, and anti-PLAP1 antibody | |
| Molecular Weight | |
| 70kDa | |
| Chromosomal Location | |
| 2q37.1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ALPP |
| Entrez Gene ID | 250 |
| Ensemble Gene ID | ENSG00000163283 |
| RefSeq Protein Accession(s) | NP_001623 |
| RefSeq mRNA Accession(s) | NM_001632 |
| RefSeq Genomic Accession(s) | NC_018913, NC_000002, NG_012189 |
| UniProt ID(s) | B2R7C7, P05187 |
| UniGene ID(s) | B2R7C7, P05187 |
| HGNC ID(s) | 439 |
| Cosmic ID(s) | ALPP |
| KEGG Gene ID(s) | hsa:250 |
| PharmGKB ID(s) | PA24730 |
| General Description of Alkaline Phosphatase / PLAP. | |
| Reacts with a 70kDa membrane-bound isozyme (Regan, Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP, shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors, can discriminate between these, other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic,, urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. | |
![Analysis of Mass Spec data (dashed-line) of fractions stained with Alkaline Phosphatase / PLAP MS-QAVA™ monoclonal antibody [Clone: ALPP/870] (solid-line), reveals that less than 4.4% of signal is attributable to non-specific binding of anti-Alkaline Phosphatase / PLAP [Clone ALPP/870] to targets other than ALPP protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-250-MSM1-P1-anti-Alkaline-Phosphatase-PLAP-antibody-150x150.png)
![Analysis of Mass Spec data (dashed-line) of fractions stained with Alkaline Phosphatase / PLAP MS-QAVA™ monoclonal antibody [Clone: PL8-F6] (solid-line), reveals that less than 4.2% of signal is attributable to non-specific binding of anti-Alkaline Phosphatase / PLAP [Clone PL8-F6] to targets other than ALPP protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-250-MSM5-P1-anti-Alkaline-Phosphatase-PLAP-antibody-150x150.png)
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