PDF DatasheetMouse Anti-Anti-CD279 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD279 Antibody Clone: J43.1
Clone J43.1 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CD279 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in PD1 / PDCD1 / CD279 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in germinal center cells in the tonsil. More moderate antibody staining intensity was present in germinal center cells in the tonsil. Low, but measureable presence of PD1 / PDCD1 / CD279 could be seen in cells in the red pulp in spleen. We were unable to detect PD1 / PDCD1 / CD279 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Anti-CD279 General Information | |
|---|---|
| Alternate Names | |
| PD1, PDCD1, CD279, PD-1, CD279, hPD-l, hSLE1, SLEB2, PD1, hPD-1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Pdcd1 |
| Entrez Gene ID | 18566 |
| Ensemble Gene ID | ENSMUSG00000026285 |
| RefSeq Protein Accession(s) | NP_032824 |
| RefSeq mRNA Accession(s) | NM_008798 |
| RefSeq Genomic Accession(s) | NC_000067 |
| UniProt ID(s) | Q02242, Q544F3 |
| UniGene ID(s) | Q02242, Q544F3 |
| Cosmic ID(s) | Pdcd1 |
| KEGG Gene ID(s) | mmu:18566 |
| General Description of Anti-CD279. | |
| The J43.1 antibody is specific for mouse CD279, also known as programmed death-1 (PD-1), a 55 kDa glycoprotein which can co-regulate T cell antigen receptor signaling and therefore modulate T cell activation. PD-1 exists in a monomeric form that is expressed by CD4- CD8- thymocytes, where it participates in the processes of clonal selection, elimination of autoreactive lymphocytes, and development of tolerance. PD-1 expression is also inducible upon activation of mature T cells, where it has been proposed to interact with the co-stimulatory receptor CD80 to limit T cell activation. Two ligands for PD-1, known as PD-L1 (B7-H1) and PD-L2 (B7-DC) are differentially expressed on T and B cells, monocytes, macrophages, NK cells or dendritic cells. PD-1 is a member of a family of receptors including CD28 and CTLA-4 (CD152), which interact with B7 ligands to provide a balance of co-stimulatory /co-inhibitory signaling important in T cell activation, tolerance, and autoimmunity.The J43.1 antibody may be used as a marker for PD-1 expression, and is commonly used for analysis of receptor-ligand interaction and function(s) in vitro and in vivo. Please choose the appropriate format for each application. | |
Selected References
Hams E, McCarron MJ, Amu S, Yagita H, Azuma M, Chen L, and Fallon PG. 2011. J. Immunol. 186:5648-5655. (in vivo blocking)Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)Brooks DG, Ha S-J, Elsaesser H, Sharpe AH, Freeman GJ, and Oldstone MBA. 2008. Proc. Natl. Acad. Sci. 105:20428-20433. (Flow cytometry)Ansari MJI, Salama AD, Chitnis T, Smith RN, Yagita H, Akiba H, Yamazaki T, Azuma M, Isai H, Khoury SJ, Auchincloss H, and Sayegh MH. 2003. J. Exp. Med. 198:63-71. (Immunohistochemistry – frozen tissue, in vivo blocking)Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubat T, Yagita H, and Honjo T. 1996. Int. Immunol. 8:765-772. (Immunoprecipitation)
Limitations and Warranty
enQuire Bio’s Mouse Anti-CD279 / PD-1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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