PDF DatasheetHuman Anti-Arginase 1 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Arginase 1 Antibody Clone: ARG1/1126
Clone ARG1/1126 Host and Isotype: Mouse IgG3 kappa
Anti-Human Arginase 1 Positive Control Sample: 293T cells. Hepatocellular Carcinoma (HCC).
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Arginase 1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Arginase 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hepatocytes in liver. More moderate antibody staining intensity was present in hepatocytes in liver. Low, but measureable presence of Arginase 1 could be seen inhematopoietic cells in the bone marrow. We were unable to detect Arginase 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Arginase 1 General Information | |
|---|---|
| Alternate Names | |
| Arginase, Arg1 | |
| Molecular Weight | |
| 35-38kDa | |
| Chromosomal Location | |
| 6q23 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ARG1 |
| Entrez Gene ID | 383 |
| Ensemble Gene ID | ENSG00000118520 |
| RefSeq Protein Accession(s) | NP_001231367, NP_000036, XP_011534103 |
| RefSeq mRNA Accession(s) | NM_000045, NM_001244438 |
| RefSeq Genomic Accession(s) | NG_007086, NC_018917, NG_031860, NC_000006 |
| UniProt ID(s) | P05089 |
| UniGene ID(s) | P05089 |
| HGNC ID(s) | 663 |
| Cosmic ID(s) | ARG1 |
| KEGG Gene ID(s) | hsa:383 |
| PharmGKB ID(s) | PA24947 |
| General Description of Arginase 1. | |
| Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine, urea. Arginase I, II are isoenzymes, which differ in subcellular localization, regulation,, possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues, granulocytes in peripheral blood. ARG-1 is a sensitive, specific marker for identification of hepatocellular carcinoma. | |
Limitations and Warranty
enQuire Bio’s Arginase 1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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