Anti-beta-Catenin Antibody Clone

$ 488.00

SKU: 1499-RBP1
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rabbit IgG
Mass Spec Validated?: Not MS Validated

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Human and Mouse Anti-beta-Catenin Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Polyclonal
Anti-beta-Catenin Antibody Clone:
Clone Host and Isotype: Rabbit IgG
Anti-Human and Mouse beta-Catenin Positive Control Sample: HeLa or MCF-7 cells. Breast carcinoma
Cellular Localization of Antibody < Staining: Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide from the middle of beta-Catenin (p120) protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

beta-Catenin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CTNNB1 antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in beta-Catenin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of beta-Catenin could be seen in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex, fibroblasts in skin, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary and Purkinje cells in the cerebellum. We were unable to detect beta-Catenin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of beta-Catenin expression as measured by anti-beta-Catenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ +++ + +++ +++ +++ +++ +++ +++ +++ ++ +++ ++ + +++ +++
CTNNB1 Variability ++ ++ ++ + + +++ ++ ++ ++ + + ++ + + ++ + ++ ++ + ++

Limitations and Warranty

enQuire Bio’s beta-Catenin Anti-Human, Mouse Polyclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-beta-Catenin Antibody Clone

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