PDF DatasheetHuman Anti-Blood Group Antigen A Antibody Product Attributes
Species: Human
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Blood Group Antigen A Antibody Clone: 3-3A
Clone 3-3A Host and Isotype: Mouse IgG1 kappa
Anti-Human Blood Group Antigen A Positive Control Sample: KG1 cells or human colorectal carcinoma tissues.
Cellular Localization of Antibody <3-3A Staining: Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Mucin isolated from an ovarian cyst fluid
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Blood Group Antigen A Previously Observed Antibody Staining Patterns
| Blood Group Antigen A General Information | |
|---|---|
| Alternate Names | |
| Histo-blood group ABO system transferase, ABO | |
| Molecular Weight | |
| Multiple | |
| Chromosomal Location | |
| 9q34.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ABO |
| Entrez Gene ID | 28 |
| Ensemble Gene ID | ENSG00000281879, ENSG00000175164 |
| RefSeq Protein Accession(s) | NP_065202 |
| RefSeq mRNA Accession(s) | NM_020469 |
| RefSeq Genomic Accession(s) | NW_009646201, NG_006669, NC_018920, NC_000009 |
| UniProt ID(s) | A0A089QDC1, P16442 |
| UniGene ID(s) | A0A089QDC1, P16442 |
| HGNC ID(s) | 79 |
| Cosmic ID(s) | ABO |
| KEGG Gene ID(s) | hsa:28 |
| PharmGKB ID(s) | PA24415 |
| General Description of Blood Group Antigen A. | |
| This MAb preferably reacts with determinants of chain A, H type 3(Gal1-3GalNAc-R), 4 (Gal1-3GalNAc-R), but not with type 1, 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This MAb is applicable for tissue staining in tumor patients with blood groups A, AB. It shows a highly heterogeneous reactivity in human colon tumor tissue, adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids, proteins detected on erythrocytes, certain epithelial cells. The A, B, H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids, glycoproteins. They are usually mucin-type,, are detected on erythrocytes, certain epithelial cells,, in secretions of certain individuals. Sixteen genetically, biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y,, precursor type 1 chain antigens. | |
Limitations and Warranty
enQuire Bio’s Blood Group Antigen A Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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