PDF DatasheetHuman Anti-Blood Group Antigen B Antibody Product Attributes
Species: Human
Tested Applications: Agglutination, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Agglutination, Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Blood Group Antigen B Antibody Clone: HEB-20
Clone HEB-20 Host and Isotype: Mouse IgM kappa
Anti-Human Blood Group Antigen B Positive Control Sample: KG1 cells or human colorectal carcinoma tissues.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Mixture of erythrocytes of group B, glycoprotein fraction isolated from saliva of secretors with blood group B
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Blood Group Antigen B Previously Observed Antibody Staining Patterns
| Blood Group Antigen B General Information | |
|---|---|
| Alternate Names | |
| Histo-blood group ABO system transferase, ABO | |
| Molecular Weight | |
| Multiple | |
| Chromosomal Location | |
| 9q34.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ABO |
| Entrez Gene ID | 28 |
| Ensemble Gene ID | ENSG00000281879, ENSG00000175164 |
| RefSeq Protein Accession(s) | NP_065202 |
| RefSeq mRNA Accession(s) | NM_020469 |
| RefSeq Genomic Accession(s) | NW_009646201, NG_006669, NC_018920, NC_000009 |
| UniProt ID(s) | A0A089QDC1, P16442 |
| UniGene ID(s) | A0A089QDC1, P16442 |
| HGNC ID(s) | 79 |
| Cosmic ID(s) | ABO |
| KEGG Gene ID(s) | hsa:28 |
| PharmGKB ID(s) | PA24415 |
| General Description of Blood Group Antigen B. | |
| The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity, reactivity to standard reagent using >5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes, vascular epithelium of blood group B controls, no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B, AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids, proteins detected on erythrocytes, certain epithelial cells. The A, B, H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids, glycoproteins. They are usually mucin type,, are detected on erythrocytes, certain epithelial cells,, in secretions of certain individuals. Sixteen genetically, biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y,, precursor type 1 chain antigens. | |
Limitations and Warranty
enQuire Bio’s Blood Group Antigen B Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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