PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Caldesmon Information for Pathologists
Summary:
Caldesmon binds calcium, calmodulin, tropomyosin and actin and regulates smooth muscle contraction (Int J Biochem Cell Biol 1997;29:1047). Regulates smooth muscle contraction and relaxation. May play a role in preventing unwanted contractions (Am J Physiol Heart Circ Physiol 2009;297:H1930). Interpretation: cytoplasmic staining. h-caldesmon is high molecular weight isoform, found in smooth muscle cells; l-caldesmon isoform is distributed in non-muscle cells (J Biol Chem 1991;266:355).
Common Uses By Pathologists:
Very useful to distinguish smooth muscle cells / lesions (positive) from myofibroblastic cells / lesions (negative or only a few cells positive, Am J Clin Pathol 2000;114:746, Am J Dermatopathol 2006;28:105). Distinguish uterine leiomyoma or leiomyosarcoma (positive) from endometrial stromal tumors (negative, Am J Surg Pathol 2001;25:455), but endometrial stromal tumors may have focal smooth muscle differentiation (Am J Surg Pathol 2002;26:1142). Sensitive and specific for epithelioid peritoneal mesothelioma (positive) versus ovarian serous carcinoma (negative, Am J Surg Pathol 2007;31:1139) and epithelioid pleural mesothelioma (positive) versus pulmonary adenocarcinoma (negative, Am J Surg Pathol 2006;30:463). Microscopic (histologic) images Images hosted on other servers:.
| Caldesmon General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 93.2 kDa | |
| Chromosomal Location | |
| q33 [chr: 7] [chr_start: 134744252] [chr_end: 134970729] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CALD1 |
| Entrez Gene ID | 800 |
| RefSeq Protein Accession(s) | XP_016868140; NP_149129; NP_149130; NP_149131; NP_149347; NP_004333; XP_016868141 |
| RefSeq mRNA Accession(s) | XR_001744877; XR_927535; XR_927537; NM_004342; NM_033140; XR_002956490; XM_024446942; XR_001744880; XM_017012650; XM_017012654; XR_927541; XM_017012652; XR_001744879; XR_002956489; XM_024446943; XR_001744881; XR_002956488; XR_927542; NM_033138; NM_033139; NM_033157 |
| RefSeq Genomic Accession(s) | NC_000007; NG_029186 |
| UniProt ID(s) | Q05682 |
| PharmGKB ID(s) | PA26034 |
| KEGG Gene ID(s) | hsa:800 |
| General Description of Caldesmon . | |
| This antibody is specific to high molecular weight (120-150 kDa) h-caldesmon. Caldesmon is involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified. The h-caldesmon variant is predominantly expressed in smooth muscle, whereas l-caldesmon (71-80 kD) is found in non-muscle tissue and cells. | |
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