PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
CD15/Granulocyte Associated Information for Pathologists
Summary:
CD15 is a A carbohydrate (most traditional diagnostic antigens are proteins) that is widely used for diagnosis of Hodgkin lymphoma. CD15 is also known as LeuM1 or Lewis X. Normal physiological roles for CD15 include mediating phagocytosis and chemotaxis of immune cells.
Notable Clinical Features:
Acute promyelocytic leukemia CD15+ staining may indicate poor prognosis. CD15 may be useful to differentiate recent injuries from post mortem damage. (Int J Legal Med 2013;127:957). In addition to its role in Hodgkin’s diagnosis, CD15 may differentiate pulmonary adenocarcinoma (CD15+) from mesothelioma (CD15-), although other markers are more specific. It also may help define neural stem cells.
Common Uses By Pathologists:
For Hodgkin lymphoma CD15 staining is used to confirm diagnosis and differentiate Hodgkin lymphoma from anaplastic large cell lymphomas. Staining is generally membranous or diffuse cytoplasmic and is observed in the golgi of Reed-Sternberg cells.
| CD15/Granulocyte Associated General Information | |
|---|---|
| Alternate Names | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Carbohydrate Antigen |
| General Description of CD15/Granulocyte Associated . | |
| This antibody reacts with a 220 kDa protein. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is also expressed in Reed-Sternberg cells and some epithelial cells. CD15 is very useful in the identification of Hodgkin disease. | |
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